Linked Life Data

2847955

Source:http://linkedlifedata.com/resource/pubmed/id/2847955

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1988-12-30
pubmed:abstractText
Acid extracts of individual pars intermedia from the Australian lungfish, Neoceratodus forsteri, were fractionated by gel filtration chromatography and analyzed for alpha-melanotropin (alpha-MSH) immunoreactivity. In these studies a C-terminal-specific alpha-MSH radioimmunoassay (RIA) was used. Following gel filtration chromatography on a Sephadex G-75 column, a major peak of immunoreactive alpha-MSH-sized material was detected. On the average there was 338 +/- 72 pmol (SD) of immunoreactive alpha-MSH per lungfish pars intermedia (n = 3). Following gel filtration the immunoreactive alpha-MSH was further analyzed by reverse-phase high-performance liquid chromatography (HPLC). Three peaks of immunoreactivity were detected. These peaks were designated Peaks 1, 2, and 3. The retention times of these peaks corresponded to, respectively, mammalian ACTH(1-13)amide, N-acetyl-ACTH(1-13)-amide, and N,O-diacetyl-ACTH(1-13)amide. Peaks 2 and 3 represented approximately 95% of the immunoreactive alpha-MSH recovered. Analysis of immunoreactive Peaks 2 and 3 by cation-ion-exchange indicated that both peaks had a net charge of +3 at pH 2.5. Since O-acetyl groups are sensitive to high pH, Peak 3 was incubated for 1 hr at 37 degrees in 0.01 N NaOH, pH 12. Under these conditions, Peak 3 eluted with the same retention time as untreated Peak 2. Collectively, these results indicate that Peaks 2 and 3 correspond to mono- and diacetylated lungfish alpha-MSH, respectively.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0016-6480
pubmed:author
pubmed:issnType
Print
pubmed:volume
71
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
468-74
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Immunological evidence for multiple forms of alpha-melanotropin (alpha-MSH) in the pars intermedia of the Australian lungfish, Neoceratodus forsteri.
pubmed:affiliation
University of Denver, Department of Biological Sciences, Colorado 80208.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't