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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1988-12-2
|
| pubmed:abstractText |
We have cloned the Shv-1 beta-lactamase gene from the R1010 plasmid into pACYC184. By subcloning and transposon mutagenesis we have localized the gene to a 1.6 kb BscI-SalI fragment of R1010, which is present in the recombinant plasmid pUB8. A 900 bp PstI fragment of pUB8 was shown to be a specific hybridization probe by testing against plasmids which encode 17 different beta-lactamase enzymes. A comparison was made of the sensitivity of the Shv-1 probe labelled with either [35S]dCTP or with photobiotin.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0022-1287
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
134
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
835-40
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:2846754-Cloning, Molecular,
pubmed-meshheading:2846754-DNA Probes,
pubmed-meshheading:2846754-DNA Transposable Elements,
pubmed-meshheading:2846754-Escherichia coli,
pubmed-meshheading:2846754-Genes, Bacterial,
pubmed-meshheading:2846754-Genetic Markers,
pubmed-meshheading:2846754-Mutation,
pubmed-meshheading:2846754-Nucleic Acid Hybridization,
pubmed-meshheading:2846754-beta-Lactamases
|
| pubmed:year |
1988
|
| pubmed:articleTitle |
Molecular cloning of the Shv-1 beta-lactamase gene and construction of an Shv-1 hybridization probe.
|
| pubmed:affiliation |
School of Biological Sciences, University of East Anglia, Norfolk, UK.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|