Linked Life Data

2846334

Source:http://linkedlifedata.com/resource/pubmed/id/2846334

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1988-12-22
pubmed:abstractText
The localization of calmodulin kinase II (CaM kinase) was studied in the retina by light and electron microscopic immunocytochemistry, and by enzymatic and immunoblot assay of cellular and subcellular tissue fractions. By light microscopy, both mono- and polyclonal antibodies revealed CaM kinase-like immunoreactivity in the inner and outer plexiform regions (synaptic layers), retinal pigment epithelium (RPE), and ganglion cells. The inner nuclear layer and photoreceptor outer segments stained much less intensely, and the outer nuclear layer did not stain. Electron microscopy confirmed the high concentration of immunoreactive protein in RPE and minimal outer segment staining. In addition, photoreceptor inner segments also contained CaM kinase-like immunoreactivity. Calcium and calmodulin stimulated phosphate incorporation into proteins of retinal cytosol and of isolated and cultured RPE. Calcium- and calmodulin-dependent kinase activity was present to a lesser degree in crude nuclei and synaptic membranes and was absent in isolated rod outer segments. Immunoblot analyses were consistent with enzymatic assays and immunocytochemistry. These data suggest that retinal CaM kinase is ideally located to play an important role in synaptic transmission and modulation of visual processes. Furthermore, its presence in RPE implies that CaM kinase may have a more ubiquitous role in regulating cellular processes than was previously recognized.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0014-4835
pubmed:author
pubmed:issnType
Print
pubmed:volume
47
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
391-402
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Localization of retinal calmodulin kinase.
pubmed:affiliation
Department of Neuroscience, UCLA School of Medicine 90024.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.