pubmed-article:2845651 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:2845651 | lifeskim:mentions | umls-concept:C1623048 | lld:lifeskim |
pubmed-article:2845651 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
pubmed-article:2845651 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
pubmed-article:2845651 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
pubmed-article:2845651 | lifeskim:mentions | umls-concept:C0441472 | lld:lifeskim |
pubmed-article:2845651 | lifeskim:mentions | umls-concept:C0442805 | lld:lifeskim |
pubmed-article:2845651 | lifeskim:mentions | umls-concept:C0333467 | lld:lifeskim |
pubmed-article:2845651 | lifeskim:mentions | umls-concept:C1159521 | lld:lifeskim |
pubmed-article:2845651 | lifeskim:mentions | umls-concept:C0918012 | lld:lifeskim |
pubmed-article:2845651 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
pubmed-article:2845651 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
pubmed-article:2845651 | pubmed:issue | 2 | lld:pubmed |
pubmed-article:2845651 | pubmed:dateCreated | 1988-11-14 | lld:pubmed |
pubmed-article:2845651 | pubmed:abstractText | The VSV-specific increase in hexose transport by BHK cells has been measured by assay of the [3H]dGlc/[14C]AIB uptake ratio. The effect was abolished by uv-irradiation of the virus, indicating that viral gene expression is required. Cells infected with the T1026 R1 mutant of VSV, which causes only slight cytopathic changes, exhibited only a slight increase in hexose uptake. Cells infected with temperature-sensitive (ts) mutants of VSV that are defective in the function of the viral N, NS, G, or M proteins at the restrictive temperature (39.5 degrees) exhibited increased [3H]dGLC/[14C]AIB uptake ratios typical of wild-type virus at either restrictive (39.5 degrees) or permissive temperature (34 degrees). Cells infected with a mutant defective in the function of the viral L protein exhibited an increased [3H]dGlc/[14C]AIB uptake ratio at permissive temperature (34 degrees) only; at restrictive temperature (39.5 degrees) the uptake ratio was essentially the same as that of mock-infected cells. Temperature-shift experiments indicated that the effect on hexose transport persisted for at least 6 hr in cells which no longer expressed function L protein, and that when expression of L was restricted to the first 2 hr of infection, an almost complete stimulation of hexose transport was observed 4 hr later. These results indicate that expression of the L gene is a necessary factor for inducing an increased hexose uptake in VSV-infected BHK cells. They also suggest that the action of the L protein on hexose transport is indirect, and is presumably mediated by other cellular constituents. The studies support the concept that an increased dGlc uptake may be a useful index of the cytopathic consequences of virus infection. | lld:pubmed |
pubmed-article:2845651 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2845651 | pubmed:language | eng | lld:pubmed |
pubmed-article:2845651 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2845651 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:2845651 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2845651 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2845651 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2845651 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2845651 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2845651 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2845651 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2845651 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2845651 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2845651 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:2845651 | pubmed:month | Oct | lld:pubmed |
pubmed-article:2845651 | pubmed:issn | 0042-6822 | lld:pubmed |
pubmed-article:2845651 | pubmed:author | pubmed-author:WidnellC CCC | lld:pubmed |
pubmed-article:2845651 | pubmed:author | pubmed-author:PasternakC... | lld:pubmed |
pubmed-article:2845651 | pubmed:author | pubmed-author:Whitaker-Dowl... | lld:pubmed |
pubmed-article:2845651 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:2845651 | pubmed:volume | 166 | lld:pubmed |
pubmed-article:2845651 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:2845651 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:2845651 | pubmed:pagination | 379-86 | lld:pubmed |
pubmed-article:2845651 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
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pubmed-article:2845651 | pubmed:year | 1988 | lld:pubmed |
pubmed-article:2845651 | pubmed:articleTitle | Stress-induced increase of hexose transport as a novel index of cytopathic effects in virus-infected cells: role of the L protein in the action of vesicular stomatitis virus. | lld:pubmed |
pubmed-article:2845651 | pubmed:affiliation | Department of Biochemistry, St. George's Hospital Medical School, London, United Kingdom. | lld:pubmed |
pubmed-article:2845651 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:2845651 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
pubmed-article:2845651 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
pubmed-article:2845651 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |