Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
19
pubmed:dateCreated
1988-11-21
pubmed:databankReference
pubmed:abstractText
Two overlapping cDNA clones encoding human DNA topoisomerase II were identified by two independent methods. In one, a human cDNA library in phage lambda was screened by hybridization with a mixed oligonucleotide probe encoding a stretch of seven amino acids found in yeast and Drosophila DNA topoisomerase II; in the other, a different human cDNA library in a lambda gt11 expression vector was screened for the expression of antigenic determinants that are recognized by rabbit antibodies specific to human DNA topoisomerase II. The entire coding sequences of the human DNA topoisomerase II gene were determined from these and several additional clones, identified through the use of the cloned human TOP2 gene sequences as probes. Hybridization between the cloned sequences and mRNA and genomic DNA indicates that the human enzyme is encoded by a single-copy gene. The location of the gene was mapped to chromosome 17q21-22 by in situ hybridization of a cloned fragment to metaphase chromosomes and by hybridization analysis with a panel of mouse-human hybrid cell lines, each retaining a subset of human chromosomes.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2845399-1195397, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845399-2427489, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845399-2580642, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845399-2824504, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845399-2838070, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845399-2886047, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845399-2985283, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845399-2985625, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845399-2985626, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845399-2986908, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845399-2992360, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845399-2999974, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845399-3000380, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845399-3006050, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845399-3012525, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845399-3014661, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845399-3016287, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845399-3017975, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845399-3023070, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845399-3031051, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845399-3034331, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845399-3034598, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845399-3040264, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845399-3313277, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845399-3344219, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845399-3456488, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845399-3755548, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845399-6090122, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845399-6219389, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845399-6291150, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845399-6323017, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845399-6326134, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845399-6589608, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845399-7273954, http://linkedlifedata.com/resource/pubmed/commentcorrection/2845399-822353
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
85
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7177-81
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Cloning and sequencing of cDNA encoding human DNA topoisomerase II and localization of the gene to chromosome region 17q21-22.
pubmed:affiliation
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't