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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
18
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pubmed:dateCreated |
1988-11-21
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pubmed:abstractText |
The promoter of mouse DNA polymerase beta gene was analyzed by combining 5'-upstream region of this gene with chloramphenicol acetyltransferase (CAT) gene and by introduction of the recombinant plasmid DNA into mouse NIH/3T3 cells. Serial deletion of the mouse DNA sequence revealed that the promoter function resides within a 33 base pair region from the nucleotide position -48 to -15 with respect to the transcription initiation site, and is highly active without enhancer sequence. The promoter region was separated into two subregions: one (-48 to -35) contains a GC-box and the other contains a 10 base pair palindrome, whose sequence is similar to one of promoter consensus sequences found in a number of promoters including adenovirus promoters. The DNA polymerase beta promoter-directed CAT expression was competitively inhibited by the simultaneous transfection of plasmid DNA containing SV40 early promoter sequence. The viral sequences which are competitive to the GC-box of DNA polymerase beta gene promoter were the GC-boxes of SV40 promoter. Therefore, it is concluded that transcription of mouse DNA polymerase beta gene is regulated by mouse trans-acting factors equivalent to human Sp1 which is known to be trans-acting protein factor acting on SV40 GC-box sequences.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2845359-1245262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2845359-240845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2845359-2427926,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2845359-2828166,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2845359-287074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2845359-2885756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2845359-3025636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2845359-3027570,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2845359-3028247,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2845359-3288540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2845359-3527815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2845359-3600656,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2845359-3614200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2845359-3945313,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2845359-4041012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2845359-4075392,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2845359-4075400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2845359-4854913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2845359-503866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2845359-6148343,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2845359-6235151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2845359-6246934,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2845359-6259538,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2845359-6267479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2845359-6296253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2845359-6301687,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2845359-6313230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2845359-6321158,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2845359-6355128,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2845359-6409418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2845359-6852040,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2845359-6960240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2845359-7217221,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2845359-944632
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
26
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
8773-87
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2845359-Animals,
pubmed-meshheading:2845359-Base Sequence,
pubmed-meshheading:2845359-Binding, Competitive,
pubmed-meshheading:2845359-DNA Mutational Analysis,
pubmed-meshheading:2845359-DNA Polymerase I,
pubmed-meshheading:2845359-Gene Expression Regulation,
pubmed-meshheading:2845359-Mice,
pubmed-meshheading:2845359-Promoter Regions, Genetic,
pubmed-meshheading:2845359-Simian virus 40,
pubmed-meshheading:2845359-Transcription, Genetic,
pubmed-meshheading:2845359-Transcription Factors
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pubmed:year |
1988
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pubmed:articleTitle |
Mouse DNA polymerase beta gene promoter: fine mapping and involvement of Sp1-like mouse transcription factor in its function.
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pubmed:affiliation |
Laboratory of Cell Biology, Aichi Cancer Center Research Institute, Nagoya, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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