Linked Life Data

2845105

Source:http://linkedlifedata.com/resource/pubmed/id/2845105

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1988-11-22
pubmed:abstractText
We characterized sarcolemmal enzymes, Na+-Ca2+ exchange, Ca2+ efflux, and Ca2+ binding in the neonatal rabbit heart. Sarcolemmal vesicles were isolated by differential and sucrose gradient centrifugation. The sarcolemmal purification factor for K+-p-nitro-phenylphosphatase (pNPPase) was 38.8 and 34.5 in the newborn and the adult, respectively. Contamination by mitochondria and sarcoplasmic reticulum was minimal. Specific activities of Na+-K+ ATPase and K+-pNPPase in the newborn were significantly lower than those in the adult. In the newborn, maximal Na+-Ca2+ exchange was 24.6 +/- 1.1 nmol Ca2+/mg/1.5 s, passive Ca2+ efflux rate was 14.6 +/- 2.2 nmol Ca2+/mg/min and Ca2+ binding was 69.4 +/- 3.3 nmol Ca2+/mg/4 min. These values were not significantly different from the adult values. At pH 6.0, Na+-Ca2+ exchange and Ca2+ binding were approximately 60% of control values (pH = 7.4) in two age groups. These values were stimulated at pH 8.0 and 9.0. In conclusion, Na+-Ca2+ exchange, Ca2+ efflux, passive Ca2+ binding and the effect of pH on Na+-Ca2+ exchange and Ca2+ binding in the newborn were similar to those in the adult.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0022-2828
pubmed:author
pubmed:issnType
Print
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
585-91
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Sarcolemmal calcium kinetics in the neonatal heart.
pubmed:affiliation
Department of Pediatrics, University of California, Los Angeles 90024.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't