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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1988-10-21
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pubmed:abstractText |
Transmembrane signal transduction was investigated in four Dictyostelium discoideum mutants that belong to the fgd A complementation group. The results show the following. (a) Cell surface cAMP receptors are present in fgd A mutants, but cAMP does not induce any of the intracellular responses, including the activation of adenylate or guanylate cyclase and chemotaxis. (b) cAMP induces down-regulation and the covalent modification (presumably phosphorylation) of the cAMP receptor. (c) The inhibitory effects of GTP gamma S and GDP beta S on cAMP binding are reduced; the stimulatory effect of cAMP on GTP gamma S binding is lost in fgd A mutants. (d) Basal high-affinity GTPase activity is reduced 40% and the stimulatory effect of cAMP is decreased from 40% in wild type to 30% in fgd A. (e) GTP-mediated stimulation and inhibition of adenylate cyclase is normal in mutant membranes. The results suggest a defective interaction between cell surface cAMP receptors and a specific G-protein in fgd A mutants. This interaction appears to be essential for nearly all signal transduction pathways in Dictyostelium discoideum.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2843545,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2843545-2821029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2843545-2835261,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2843545-2864084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2843545-2871026,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2843545-2892530,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2843545-2893972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2843545-2982884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2843545-2988543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2843545-3025212,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2843545-3039908,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2843545-3100295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2843545-3771567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2843545-3793759,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2843545-4314777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2843545-6092374,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2843545-6093807
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenylate Cyclase,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/GTP Phosphohydrolases,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Guanosine 5'-O-(3-Thiotriphosphate),
http://linkedlifedata.com/resource/pubmed/chemical/Guanosine Diphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Guanosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Thionucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/guanosine 5'-O-(2-thiodiphosphate)
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0021-9525
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
107
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
521-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2843545-Adenylate Cyclase,
pubmed-meshheading:2843545-Binding, Competitive,
pubmed-meshheading:2843545-Chemotaxis,
pubmed-meshheading:2843545-Cyclic AMP,
pubmed-meshheading:2843545-Dictyostelium,
pubmed-meshheading:2843545-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2843545-GTP Phosphohydrolases,
pubmed-meshheading:2843545-GTP-Binding Proteins,
pubmed-meshheading:2843545-Guanosine 5'-O-(3-Thiotriphosphate),
pubmed-meshheading:2843545-Guanosine Diphosphate,
pubmed-meshheading:2843545-Guanosine Triphosphate,
pubmed-meshheading:2843545-Immunoassay,
pubmed-meshheading:2843545-Kinetics,
pubmed-meshheading:2843545-Mutation,
pubmed-meshheading:2843545-Receptors, Cyclic AMP,
pubmed-meshheading:2843545-Thionucleotides
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pubmed:year |
1988
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pubmed:articleTitle |
Signal transduction in Dictyostelium fgd A mutants with a defective interaction between surface cAMP receptors and a GTP-binding regulatory protein.
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pubmed:affiliation |
Department of Biology, University of Leiden, The Netherlands.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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