Linked Life Data

2843525

Source:http://linkedlifedata.com/resource/pubmed/id/2843525

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
27
pubmed:dateCreated
1988-10-19
pubmed:databankReference
pubmed:abstractText
NAD(P)H:menadione oxidoreductase (NMOR1) is a flavoprotein that catalyzes the two-electron reduction of various redox dyes and quinones. It has been proposed that this enzyme may have a protective effect against cancer caused by quinones and their metabolic precursors. We show that tetrachlorodibenzo-p-dioxin (TCDD) treatment of the human hepatoblastoma cell line Hep-G2 produces a 5-fold induction of NMOR activity. Several overlapping human NMOR1 cDNAs were isolated from a human liver lambda gt 11 expression library, and their composite sequence corresponds to an mRNA of 2448 nucleotides containing a continuous open reading frame encoding a protein of 274 residues (molecular weight, 30,880). The corresponding human NMOR1 mRNA has an unusually long 3'-untranslated region (1679 base pairs) with four potential polyadenylation signals (I-IV) at positions 986, 1460, 1838, and 2419 and a single copy of human Alu repetitive sequence between polyadenylation sites II and III. Southern blot analysis of human genomic DNA suggests the presence of a single NMOR1 gene approximately 10 kilobases (KB) in length. The use of three of the aforementioned polyadenylation signals is likely to account for the three different species (2.7, 1.7, and 1.2 kb) of mRNA hybridizing to NMOR1 cDNA in Hep-G2 cells. Indeed several partial cDNA clones were isolated that corresponded to the mRNA derived by use of the proximal polyadenylation signal. Interestingly, the longest (2.7 kb) mRNA species was induced severalfold by TCDD, whereas the other two mRNAs (1.7 and 1.2 kb) were induced to a much lesser extent by TCDD treatment. The human NMOR1 cDNA and protein are 83 and 85% similar to rat liver cytosolic NMOR1 cDNA and protein, respectively. Southern analysis of DNA from 54 human x mouse and 39 human x hamster somatic cell hybrids shows that the NMOR1 gene resides on human chromosome 16.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
263
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
13572-8
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:2843525-Amino Acid Sequence, pubmed-meshheading:2843525-Animals, pubmed-meshheading:2843525-Base Sequence, pubmed-meshheading:2843525-Carcinoma, Hepatocellular, pubmed-meshheading:2843525-Chromosome Mapping, pubmed-meshheading:2843525-Chromosomes, Human, Pair 16, pubmed-meshheading:2843525-Codon, pubmed-meshheading:2843525-Cytosol, pubmed-meshheading:2843525-DNA, pubmed-meshheading:2843525-DNA Restriction Enzymes, pubmed-meshheading:2843525-Dioxins, pubmed-meshheading:2843525-Enzyme Induction, pubmed-meshheading:2843525-Humans, pubmed-meshheading:2843525-Liver Neoplasms, pubmed-meshheading:2843525-Molecular Sequence Data, pubmed-meshheading:2843525-NAD(P)H Dehydrogenase (Quinone), pubmed-meshheading:2843525-Nucleic Acid Hybridization, pubmed-meshheading:2843525-Quinone Reductases, pubmed-meshheading:2843525-RNA, Messenger, pubmed-meshheading:2843525-Tetrachlorodibenzodioxin, pubmed-meshheading:2843525-Tumor Cells, Cultured
pubmed:year
1988
pubmed:articleTitle
Human dioxin-inducible cytosolic NAD(P)H:menadione oxidoreductase. cDNA sequence and localization of gene to chromosome 16.
pubmed:affiliation
Department of Cell Biology, New York University Medical Center, New York 10016.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.