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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1979-5-26
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pubmed:abstractText |
Partially purified adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] from bovine brain cortex was fractionated into two separate forms by calcium-dependent regulatory protein (CDR)-Sepharose affinity chromatography. The major form of the enzyme, comprising approximately 80% of the applied activity, did not bind to the affinity column in the presence of Ca2+ and was insensitive to the CDR. Approximately 20% of adenylate cyclase activity was absorbed to CDR-Sepharose in the presence of Ca2+. This activity was stimulated by Ca2+ and CDR. This study directly demonstrates that brain cortex contains Ca2+-CDR-sensitive and -insensitive forms of adenylate cyclase and indicates that CDR-Sepharose may be a useful tool for purification of adenylate cyclase. The Ca2+ -stimulated adenylate cyclase was purified at least 55-fold with a 13% yield.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/284333-1069993,
http://linkedlifedata.com/resource/pubmed/commentcorrection/284333-1120776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/284333-164029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/284333-165665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/284333-167852,
http://linkedlifedata.com/resource/pubmed/commentcorrection/284333-170936,
http://linkedlifedata.com/resource/pubmed/commentcorrection/284333-180424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/284333-187190,
http://linkedlifedata.com/resource/pubmed/commentcorrection/284333-188813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/284333-198799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/284333-201280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/284333-205796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/284333-206539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/284333-208465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/284333-272673,
http://linkedlifedata.com/resource/pubmed/commentcorrection/284333-407229,
http://linkedlifedata.com/resource/pubmed/commentcorrection/284333-4197911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/284333-4313941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/284333-4348576,
http://linkedlifedata.com/resource/pubmed/commentcorrection/284333-4357877,
http://linkedlifedata.com/resource/pubmed/commentcorrection/284333-4367813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/284333-4368466,
http://linkedlifedata.com/resource/pubmed/commentcorrection/284333-4453188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/284333-4827395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/284333-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/284333-603028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/284333-627551,
http://linkedlifedata.com/resource/pubmed/commentcorrection/284333-814900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/284333-833154,
http://linkedlifedata.com/resource/pubmed/commentcorrection/284333-947908
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
76
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
204-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1979
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pubmed:articleTitle |
Resolution of adenylate cyclase sensitive and insensitive to Ca2+ and calcium-dependent regulatory protein (CDR) by CDR-sepharose affinity chromatography.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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