Linked Life Data

2843296

Source:http://linkedlifedata.com/resource/pubmed/id/2843296

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1988-10-25
pubmed:abstractText
We describe here for the first time the isolation of a yeast nuclear scaffold that maintains specific interactions with yeast genomic DNA sequences. The scaffold-DNA interaction is reversible and saturable, and some binding sites are conserved between yeast and Drosophila KC cells. Second, we find that the specific sequences bound to the yeast nuclear scaffold are the putative origins of replication (ARS elements) and a chromosomal centromere, CENIII. The scaffold association has been closely mapped at the ARS1 locus, and appears to include the 11 bp ARS consensus, but not the ABF-1 binding site. Competition studies show that ARS1 does not compete for CENIII binding, allowing us to distinguish two classes of scaffold attachment sites by functional and structural criteria.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0092-8674
pubmed:author
pubmed:issnType
Print
pubmed:day
23
pubmed:volume
54
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
967-78
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Chromosomal ARS and CEN elements bind specifically to the yeast nuclear scaffold.
pubmed:affiliation
Swiss Institute for Experimental Cancer Research, Epalinges s/Lausanne.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't