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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1988-10-25
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pubmed:abstractText |
Yeast Ty1 elements are retrotransposons that transpose via an RNA intermediate found in a virus-like particle (Ty-VLP). A Ty-encoded reverse transcriptase activity found inside the particles is capable of giving rise to full-length reverse transcripts. The predominant form of these reverse transcripts is a full-length linear duplex DNA. We have developed a cell-free system for transposition of Ty1 DNA molecules into a bacteriophage lambda target. Purified Ty-VLPs and target DNA are the only macromolecular components required for the transposition reaction. A TYB-encoded protein, p90-TYB, contains amino acid sequences that are similar to those of retroviral integrase proteins. Mutations in the integrase coding region abolish transposition both in vivo and in vitro.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Circular,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Fungal,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Recombinant,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Nucleotidyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Transposable Elements,
http://linkedlifedata.com/resource/pubmed/chemical/Integrases
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
23
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pubmed:volume |
54
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
955-66
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:2843295-Antibodies, Monoclonal,
pubmed-meshheading:2843295-Bacteriophage lambda,
pubmed-meshheading:2843295-Base Sequence,
pubmed-meshheading:2843295-Cell Fractionation,
pubmed-meshheading:2843295-Cell-Free System,
pubmed-meshheading:2843295-Centrifugation, Density Gradient,
pubmed-meshheading:2843295-DNA, Circular,
pubmed-meshheading:2843295-DNA, Fungal,
pubmed-meshheading:2843295-DNA, Recombinant,
pubmed-meshheading:2843295-DNA Nucleotidyltransferases,
pubmed-meshheading:2843295-DNA Transposable Elements,
pubmed-meshheading:2843295-Escherichia coli,
pubmed-meshheading:2843295-Integrases,
pubmed-meshheading:2843295-Mutation,
pubmed-meshheading:2843295-Plasmids,
pubmed-meshheading:2843295-Retroviridae,
pubmed-meshheading:2843295-Saccharomyces cerevisiae,
pubmed-meshheading:2843295-Sequence Homology, Nucleic Acid
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pubmed:year |
1988
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pubmed:articleTitle |
The DNA intermediate in yeast Ty1 element transposition copurifies with virus-like particles: cell-free Ty1 transposition.
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pubmed:affiliation |
Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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