2843147

Source:http://linkedlifedata.com/resource/pubmed/id/2843147

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0006556, umls-concept:C0042761, umls-concept:C0184511, umls-concept:C0184661, umls-concept:C0439831
pubmed:issue	1-2
pubmed:dateCreated	1988-9-27
pubmed:abstractText	The efficiency of viroid cloning can be increased by three to four orders of magnitude when the synthesis of viroid cDNA is primed in such a way that it carries identical sticky ends on both termini and when the multi-ion transformation is applied.
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/7506870
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/DNA, Viral, http://linkedlifedata.com/resource/pubmed/chemical/DNA Restriction Enzymes
pubmed:status	MEDLINE
pubmed:issn	0304-8608
pubmed:author	pubmed-author:PuchtaHH, pubmed-author:SängerH LHL
pubmed:issnType	Print
pubmed:volume	101
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	137-40
pubmed:dateRevised	2006-11-15
pubmed:meshHeading	pubmed-meshheading:2843147-Base Sequence, pubmed-meshheading:2843147-Cloning, Molecular, pubmed-meshheading:2843147-DNA, Viral, pubmed-meshheading:2843147-DNA Restriction Enzymes, pubmed-meshheading:2843147-Efficiency, pubmed-meshheading:2843147-Escherichia coli, pubmed-meshheading:2843147-Transformation, Genetic, pubmed-meshheading:2843147-Viroids
pubmed:year	1988
pubmed:articleTitle	An improved procedure for the rapid one-step-cloning of full-length viroid cDNA.
pubmed:affiliation	Max-Planck-Institut für Biochemie, Abteilung Viroidforschung, Martinsried bei München, Federal Republic of Germany.
pubmed:publicationType	Journal Article