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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
16
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pubmed:dateCreated |
1988-10-5
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pubmed:abstractText |
The optional group I intron of the mitochondrial 21S rRNA gene of Saccharomyces cerevisiae contains a 235-codon-long open reading frame the translation product of which (the omega transposase) catalyzes the formation of a double-strand break within the intron-minus (omega-) copies of the same gene. Purified omega transposase generates in vitro a 4-base-pair staggered cut with 3' hydroxyl overhangs at the exact position where the intron eventually inserts in the gene. Using randomly mutagenized synthetic oligonucleotides, single-base mutants were produced at 21 positions around the cleavage site. Experiments with these oligonucleotides show that the recognition site extends over an 18-base pair-long sequence within which minimal sequence degeneracy is tolerated. The intron-encoded omega transposase is, therefore, one of the most specific restriction endonucleases known to date.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2842757-11894905,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2842757-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2842757-3004738,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2842757-3020559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2842757-3033611,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2842757-3886160,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2842757-3886163,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2842757-3886164,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2842757-6156002,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2842757-6246368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2842757-6273866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2842757-6300094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2842757-6301692,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2842757-6310323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2842757-6313222,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2842757-6361491,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2842757-6380756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2842757-6397306,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2842757-6817818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2842757-765750
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
85
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6022-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1988
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pubmed:articleTitle |
Recognition and cleavage site of the intron-encoded omega transposase.
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pubmed:affiliation |
Département de Biologie Moléculaire, Institut Pasteur, Paris, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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