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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1988-9-26
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pubmed:abstractText |
To determine whether epithelial ion transport abnormalities in cystic fibrosis (CF) might reflect abnormal regulation of intracellular Ca2+ levels, cytosolic free calcium (Cai2+) was measured using fura-2 or quin2 in suspensions of normal or CF nasal epithelial cells derived from primary cell culture. The basal Cai2+ level measured with fura-2 in CF nasal epithelia was 155 +/- 9 nM (n = 5), a value not significantly different from normal nasal epithelia (143 +/- 16 nM, n = 5). Total cell calcium was measured by atomic absorption spectroscopy and no differences were observed between CF (6.3 +/- 0.5 nmol/mg protein; n = 3) and normal (6.2 +/- 1.2 nmol/mg protein; n = 3) nasal epithelial cells. Placing Na+ loaded cells in a low (10 mM) extracellular Na+ solution resulted in a rapid increase in Cai2+ consistent with Ca2+ uptake via a plasmalemmal Na+-Ca2+ exchanger. The level of Cai2+ achieved by this low Na+ maneuver was not significantly different in CF cells compared to normal cells. Neither isoproterenol (10(-5) M) nor forskolin (10(-6) M) had any effect on Cai2+ in normal or CF nasal epithelial cells. Thus, it appears that differences in cell Cai2+, as measured by fluorescent chelators in suspensions of cultured cells, do not account for the abnormalities in basal or isoproterenol stimulated ion transport in CF tissues.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Aminoquinolines,
http://linkedlifedata.com/resource/pubmed/chemical/Benzofurans,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Ethers,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Fura-2,
http://linkedlifedata.com/resource/pubmed/chemical/Ionomycin,
http://linkedlifedata.com/resource/pubmed/chemical/Isoproterenol,
http://linkedlifedata.com/resource/pubmed/chemical/Ouabain,
http://linkedlifedata.com/resource/pubmed/chemical/Quin2,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0031-3998
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
24
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
79-84
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:2842726-Adolescent,
pubmed-meshheading:2842726-Adult,
pubmed-meshheading:2842726-Aged,
pubmed-meshheading:2842726-Aminoquinolines,
pubmed-meshheading:2842726-Benzofurans,
pubmed-meshheading:2842726-Calcium,
pubmed-meshheading:2842726-Cells, Cultured,
pubmed-meshheading:2842726-Child,
pubmed-meshheading:2842726-Cyclic AMP,
pubmed-meshheading:2842726-Cystic Fibrosis,
pubmed-meshheading:2842726-Cytosol,
pubmed-meshheading:2842726-Epithelium,
pubmed-meshheading:2842726-Ethers,
pubmed-meshheading:2842726-Female,
pubmed-meshheading:2842726-Fluorescent Dyes,
pubmed-meshheading:2842726-Fura-2,
pubmed-meshheading:2842726-Humans,
pubmed-meshheading:2842726-Ionomycin,
pubmed-meshheading:2842726-Isoproterenol,
pubmed-meshheading:2842726-Male,
pubmed-meshheading:2842726-Middle Aged,
pubmed-meshheading:2842726-Nasal Mucosa,
pubmed-meshheading:2842726-Ouabain,
pubmed-meshheading:2842726-Sodium,
pubmed-meshheading:2842726-Spectrophotometry, Atomic
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pubmed:year |
1988
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pubmed:articleTitle |
Cell calcium levels of normal and cystic fibrosis nasal epithelium.
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pubmed:affiliation |
National Institute of Environmental Health Sciences, Laboratory of Molecular Biophysics, Research Triangle Park, North Carolina 27709.
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pubmed:publicationType |
Journal Article
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