Linked Life Data

2842293

Source:http://linkedlifedata.com/resource/pubmed/id/2842293

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1988-10-6
pubmed:abstractText
Caulobacter crescentus incorporates two distinct, but related proteins into the polar flagellar filament: a 27-kilodalton (kDa) flagellin is assembled proximal to the hook and a 25-kDa flagellin forms the distal end of the filament. These two proteins and a third, related flagellin protein of 29 kDa are encoded by three tandem genes (alpha-flagellin cluster) in the flaEY gene cluster (S.A. Minnich and A. Newton, Proc. Natl. Acad. Sci. USA 84: 1142-1146, 1987). Since point mutations in flagellin genes had not been isolated their requirement for flagellum function and fla gene expression was not known. To address these questions, we developed a gene replacement protocol that uses cloned flagellin genes mutagenized by either Tn5 transposons in vivo or the replacement of specific DNA fragments in vitro by the antibiotic resistance omega cassette. Analysis of gene replacement mutants constructed by this procedure led to several conclusions. (i) Mutations in any of the three flagellin genes do not cause complete loss of motility. (ii) Tn5 insertions in the 27-kDa flagellin gene and a deletion mutant of this gene do not synthesize the 27-kDa flagellin, but they do synthesize wild-type levels of the 25-kDa flagellin, which implies that the 27-kDa flagellin is not required for expression and assembly of the 25-kDa flagellin; these mutants show slightly impaired motility on swarm plates. (iii) Mutant PC7810, which is deleted for the three flagellin genes in the flaEY cluster, does not synthesize the 27- or 29-kDa flagellin, and it is significantly more impaired for motility on swarm plates than mutants with defects in only the 27-kDa flagellin gene. The synthesis of essentially normal levels of 25-kDa flagellin by strain PC7810 confirms that additional copies of the 25-kDa flagellin map outside the flaEY cluster (beta-flagellin cluster) and that these flagellin genes are active. Thus, while the 29- and 27-kDa flagellins are not absolutely essential for motility in C. crescentus, their assembly into the flagellar structure is necessary for normal flagellar function.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2842293-1137965, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842293-1195397, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842293-14220656, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842293-16593248, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842293-17248770, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842293-231729, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842293-2881560, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842293-336599, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842293-3469658, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842293-3517878, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842293-4078896, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842293-6237955, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842293-6256652, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842293-6260575, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842293-6276364, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842293-6294658, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842293-6305939, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842293-6324186, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842293-6327646, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842293-6437899, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842293-6750611, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842293-690070, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842293-6949897, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842293-7037746, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842293-7056757, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842293-7237543, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842293-7338918, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842293-762024, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842293-912749
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
170
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3953-60
pubmed:dateRevised
2010-9-9
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Role of the 25-, 27-, and 29-kilodalton flagellins in Caulobacter crescentus cell motility: method for construction of deletion and Tn5 insertion mutants by gene replacement.
pubmed:affiliation
Department of Molecular Biology, Princeton University, New Jersey 08544.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't