Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1988-10-6
pubmed:abstractText
A Flavobacterium sp. (strain 50001), capable of degrading 2,4-dichlorophenoxyacetate (2,4-D), 2-methyl-4-chlorophenoxyacetate, and 2-chlorobenzoate and imparting resistance to mercury, harbored a degradative plasmid, pRC10. Cured strains of the Flavobacterium sp. lost the plasmid as well as the ability to degrade these chlorinated compounds. Comparison of this plasmid with the well-characterized 2,4-D-degradative plasmid pJP4 from Alcaligenes eutrophus showed regions of homology between the two plasmids. Restriction fragments of plasmid pRC10 which shared homology with the regions conferring 2,4-D-degradative genes (tfd) of plasmid pJP4 were cloned into a broad-host-range plasmid and studied in Pseudomonas putida. From the results obtained, the cloned DNA fragment expressed the genes for 2,4-D monooxygenase (tfdA) and 2,4-dichlorophenol hydroxylase (tfdB). In spite of the similarity in function, the size (45 kilobases) and restriction pattern of plasmid pRC10 were considerably different from those of pJP4 (80 kilobases). This may be due to the difference in the microbial background during evolution of the two plasmids.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2842290-1176436, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842290-1195397, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842290-13406225, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842290-13472508, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842290-14907713, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842290-16347272, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842290-17779620, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842290-2430049, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842290-2981813, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842290-3015870, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842290-3036764, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842290-3301806, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842290-3415221, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842290-3611023, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842290-3782038, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842290-388356, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842290-3923930, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842290-3968031, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842290-4418209, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842290-4621687, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842290-4712575, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842290-4852192, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842290-5123887, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842290-5123888, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842290-6257648, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842290-6314410, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842290-6358103, http://linkedlifedata.com/resource/pubmed/commentcorrection/2842290-672896
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
170
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3897-902
pubmed:dateRevised
2010-9-9
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Isolation and characterization of a new plasmid from a Flavobacterium sp. which carries the genes for degradation of 2,4-dichlorophenoxyacetate.
pubmed:affiliation
Institute of Food and Agricultural Sciences, University of Florida, Gainesville 32611.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't