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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1988-9-27
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pubmed:abstractText |
Quantitative two-color fluorescent analysis of Simian virus (SV40) infection of permissive CV-1 cells was investigated. Analysis included by quantitation of cellular DNA, the early viral tumor (T) antigen with a monoclonal antibody, and late viral (V) antigens with a polyclonal antibody. T antigen was detected in all phases of the cell cycle at 6 and 12 h, after SV40 infection of growth arrested cells. At later time intervals, the percentage of T-antigen-positive cells increased with the induction of the cells into successive rounds of DNA synthesis and an increase in tetraploid-polyploid cells. The amount of T antigen per cell increased as the cells entered the successive stages of the cell cycle (G0/G1----G2 + M----tetraploid S and G2 + M). The V antigen from adsorbed virus was detected immediately after infection. Synthesis of V antigen began in late S and G2 + M phases of the cell cycle. This quantitative analysis allows a definitive determination of antigen per cell in a population correlated with the cell cycle and may be useful in correlating viral and cellular events with transformation.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0196-4763
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
52-9
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:2842119-Animals,
pubmed-meshheading:2842119-Antigens, Viral, Tumor,
pubmed-meshheading:2842119-Cell Cycle,
pubmed-meshheading:2842119-Cell Line,
pubmed-meshheading:2842119-Cercopithecus aethiops,
pubmed-meshheading:2842119-DNA, Viral,
pubmed-meshheading:2842119-Flow Cytometry,
pubmed-meshheading:2842119-Fluorescent Antibody Technique,
pubmed-meshheading:2842119-Kidney,
pubmed-meshheading:2842119-Methods,
pubmed-meshheading:2842119-Simian virus 40,
pubmed-meshheading:2842119-Tumor Virus Infections
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pubmed:year |
1988
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pubmed:articleTitle |
Analysis of simian virus 40 infection of CV-1 cells by quantitative two-color fluorescence with flow cytometry.
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pubmed:affiliation |
Department of Microbiology and Immunology, Albany Medical College, New York 12208.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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