Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
24
pubmed:dateCreated
1988-9-20
pubmed:databankReference
pubmed:abstractText
At least four genes encode the human alkaline phosphatases (ALPs). The genes encoding three of these proteins (intestinal, placental, and placental-like ALPs), are linked on the long arm of chromosome 2, while the fourth gene (encoding liver/bone/kidney ALP) is located on chromosome 1. One of the linked genes, intestinal alkaline phosphatase, has been isolated on two overlapping phage clones and sequenced in its entirety. The gene is composed of 11 exons interrupted by 10 introns. Introns in intestinal, placental, and liver/bone/kidney ALPs occur at analogous positions (see accompanying articles), confirming that these genes arose from a single ancestral ALP gene. Multiple intestinal ALP mRNA species can be detected in RNA isolated from adult and fetal intestine and from cell line RNAs. In cell line RNA, the various species are the result of differential use of at least three of the four polyadenylation signals present in the intestinal ALP gene. A 125-base pair fragment located 5' to the first exon can function as a promoter in mammalian cells. This region contains two putative transcription signals, a TATA-like sequence and a consensus binding site for the transcription factor Sp1.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
263
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
12011-9
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:2841341-Alkaline Phosphatase, pubmed-meshheading:2841341-Base Sequence, pubmed-meshheading:2841341-Chromosomes, Human, Pair 2, pubmed-meshheading:2841341-DNA, pubmed-meshheading:2841341-DNA, Recombinant, pubmed-meshheading:2841341-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:2841341-Enhancer Elements, Genetic, pubmed-meshheading:2841341-Exons, pubmed-meshheading:2841341-Humans, pubmed-meshheading:2841341-Intestines, pubmed-meshheading:2841341-Introns, pubmed-meshheading:2841341-Molecular Sequence Data, pubmed-meshheading:2841341-Nucleic Acid Hybridization, pubmed-meshheading:2841341-Placenta, pubmed-meshheading:2841341-Promoter Regions, Genetic, pubmed-meshheading:2841341-RNA, Messenger, pubmed-meshheading:2841341-Sequence Homology, Nucleic Acid, pubmed-meshheading:2841341-Simian virus 40
pubmed:year
1988
pubmed:articleTitle
Sequence and characterization of the human intestinal alkaline phosphatase gene.
pubmed:affiliation
Department of Human Genetics, University of Pennsylvania, School of Medicine, Philadelphia 19104.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't