Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1988-9-20
pubmed:abstractText
Cyclic AMP has long been proposed to be the intracellular second messenger that conveys the inhibitory signal for T-cell activation and clonal T-cell proliferation. The present study further explores the mechanism by which the cAMP pathway regulates human T-lymphocyte interleukin-2 (IL-2) production and T-cell blastogenesis. Activation of adenylate cyclase, inhibition of cAMP-dependent phosphodiesterase, or the direct addition of the cell-permeable cAMP analog, 8-N3-cAMP, increased occupancy of intracellular cAMP receptors, inhibited IL-2 production, and reduced T-cell proliferation. However, inhibition of cAMP-dependent protein phosphorylation by N-[2-(methylamino)ethyl]-5-isoquinolinesulfonamide (H-8), a cell-permeable inhibitor of cyclic nucleotide-dependent protein kinase, partially restored IL-2 production. Our data support the conclusion that the cAMP pathway conveys an inhibitory signal for IL-2 production and T-cell proliferation via an integral protein phosphorylation step.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0008-8749
pubmed:author
pubmed:issnType
Print
pubmed:volume
115
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
88-99
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:2841027-4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone, pubmed-meshheading:2841027-Adenylate Cyclase, pubmed-meshheading:2841027-Adolescent, pubmed-meshheading:2841027-Adult, pubmed-meshheading:2841027-Aged, pubmed-meshheading:2841027-Azides, pubmed-meshheading:2841027-Calcimycin, pubmed-meshheading:2841027-Cell Line, pubmed-meshheading:2841027-Cyclic AMP, pubmed-meshheading:2841027-Dose-Response Relationship, Immunologic, pubmed-meshheading:2841027-Female, pubmed-meshheading:2841027-Forskolin, pubmed-meshheading:2841027-Humans, pubmed-meshheading:2841027-Immunosuppressive Agents, pubmed-meshheading:2841027-Interleukin-1, pubmed-meshheading:2841027-Interleukin-2, pubmed-meshheading:2841027-Lymphocyte Activation, pubmed-meshheading:2841027-Male, pubmed-meshheading:2841027-Middle Aged, pubmed-meshheading:2841027-Phosphorylation, pubmed-meshheading:2841027-T-Lymphocytes, pubmed-meshheading:2841027-Tetradecanoylphorbol Acetate
pubmed:year
1988
pubmed:articleTitle
Control of human T-lymphocyte interleukin-2 production by a cAMP-dependent pathway.
pubmed:affiliation
Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't