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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
15
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pubmed:dateCreated |
1988-9-7
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pubmed:abstractText |
Primary cultures of rat alveolar type II cells bind radiolabeled pulmonary surfactant protein A (SP-A) with high affinity. The binding of 125I-labeled SP-A is time- and temperature-dependent and is not accompanied by significant degradation. The binding process is saturable at low concentrations of SP-A (5 micrograms/ml), and unlabeled SP-A readily competes with labeled SP-A for cellular binding sites. Subsequent to binding, two pools of cell-associated 125I-labeled SP-A can be identified based upon sensitivity to trypsin at 0 degrees C. It is likely that the trypsin-sensitive pool comprises 125I-labeled SP-A bound to the cell surface and the trypsin-insensitive pool comprises the internalized protein. Scatchard analysis of cell surface binding of SP-A at 0.1-10 micrograms/ml shows positive cooperativity at concentrations between 0.1 and 1 micrograms/ml. Hill plots give nH = 1.34 +/- 0.08 with an apparent dissociation constant K'd = 1.02 +/- 0.32 micrograms/ml (which is 0.64 +/- 0.19 nM if the native molecular mass of oligomeric SP-A is assumed to be 1.6 MDa). The binding of SP-A to type II cells shows an absolute requirement for Ca2+. The putative receptor for SP-A is unaffected by treatment of type II cells with a variety of proteases and N-Glycanase (EC 3.5.1.52). Alveolar macrophages also exhibit high-affinity binding of SP-A, but rat lung fibroblasts and the alveolar epithelial cell line L2 exhibit only nonspecific binding.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2840667-1175787,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2840667-2425225,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2840667-2426341,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2840667-2449439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2840667-2820982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2840667-2856926,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2840667-2858220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2840667-3006655,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2840667-3021734,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2840667-3035561,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2840667-34631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2840667-3467361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2840667-3469643,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2840667-3579914,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2840667-3654429,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2840667-3755136,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2840667-3818626,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2840667-3838094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2840667-3840389,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2840667-3863100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2840667-3918569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2840667-3922400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2840667-4368448,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2840667-4616002,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2840667-4633419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2840667-4733239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2840667-5143846,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2840667-5835014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2840667-6204769,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2840667-6300903,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2840667-6321901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2840667-6547963,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2840667-6687593,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/2840667-7035455
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
85
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5566-70
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2840667-Animals,
pubmed-meshheading:2840667-Apoproteins,
pubmed-meshheading:2840667-Cell Line,
pubmed-meshheading:2840667-Cells, Cultured,
pubmed-meshheading:2840667-Fibroblasts,
pubmed-meshheading:2840667-Male,
pubmed-meshheading:2840667-Pulmonary Alveoli,
pubmed-meshheading:2840667-Pulmonary Surfactant-Associated Proteins,
pubmed-meshheading:2840667-Pulmonary Surfactants,
pubmed-meshheading:2840667-Rats,
pubmed-meshheading:2840667-Rats, Inbred Strains,
pubmed-meshheading:2840667-Receptors, Cell Surface,
pubmed-meshheading:2840667-Temperature,
pubmed-meshheading:2840667-Time Factors
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pubmed:year |
1988
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pubmed:articleTitle |
Alveolar type II cells express a high-affinity receptor for pulmonary surfactant protein A.
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pubmed:affiliation |
National Jewish Center for Immunology and Respiratory Medicine, Denver, CO 80206.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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