Linked Life Data

2840122

Source:http://linkedlifedata.com/resource/pubmed/id/2840122

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1988-9-1
pubmed:abstractText
The influence of chlorpromazine (CPZ) on the transverse mobility of spin-labeled phospholipids incorporated into human erythrocytes was investigated by electron spin resonance. The very slow transverse diffusion of phosphatidylcholine, as well as the absence of transverse mobility of sphingomyelin were not modified even by sublytic concentrations (approximately equal to 1 mM) of CPZ. On the other hand, the rapid outside-inside translocation of the aminophospholipids (Seigneuret and Devaux (1984) Proc. Natl. Acad. Sci. USA 81, 3751-3755), was slightly hindered in CPZ containing membranes. If the spin-labeled aminolipids were incorporated in erythrocytes and allowed to flip to the inner monolayer before CPZ addition, a fraction of the spin labels (10-15%) flipped back instantaneously from the inner to the outer leaflet, upon incubation with CPZ. Similar experiments carried out with spin-labeled phosphatidylcholine and spin-labeled sphingomyelin showed that a fraction of the spin-labeled choline derivatives flip instantaneously to the inner leaflet if CPZ was added after the spin labels. Addition of lysophosphatidylcholine had no effect on the spin-labeled phospholipid redistribution nor on their transmembrane mobility. We interpret the immediate effect of CPZ addition as being due to a reorganization of the bilayer accompanying the rapid CPZ membrane penetration, phenomenon which is independent of the CPZ effect on the steady-state activity of the 'aminophospholipid translocase', the latter effect being probably a direct CPZ-protein interaction. By comparison of the time course of phosphatidylserine transverse diffusion in control discocyte cells and in CPZ-induced stomatocytes, we infer that the difference in cell shape is not a major factor in the regulation of the active inward transport of aminophospholipids in human erythrocytes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
21
pubmed:volume
942
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
271-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Influence of chlorpromazine on the transverse mobility of phospholipids in the human erythrocyte membrane: relation to shape changes.
pubmed:affiliation
Institut de Biologie Physico-Chimique, Paris, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't