Linked Life Data

2839499

Source:http://linkedlifedata.com/resource/pubmed/id/2839499

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
22
pubmed:dateCreated
1988-8-24
pubmed:abstractText
The alleles of dnaA46, dnaA5, and dnaA204 have been cloned from the Escherichia coli chromosome into a protein-overproducing vector. Under conditions of induced expression, similar amounts of dnaA46 and dnaA5 gene products and less of the dnaA204 gene product were observed. Facilitated by elevated expression levels, an improved method for purification of dnaA+ protein and the purification of dnaA46 protein have been obtained. The replication activity of dnaA46 protein did not appear to be thermolabile upon prior incubation at various temperatures. Thermolabile replication activity was observed under conditions of coupled DNA synthesis. In contrast with the wild type protein, DNA synthesis dependent on dnaA46 protein showed a pronounced lag before incorporation of deoxyribonucleotides.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
263
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
10625-32
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Purification and characterization of the dnaA46 gene product.
pubmed:affiliation
Department of Biochemistry, Michigan State University, East Lansing 48824.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.