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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1988-7-29
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pubmed:abstractText |
T-cell activation induces expression of the hematopoietic growth factor granulocyte-macrophage colony-stimulating factor (GM-CSF). To define the molecular events involved in the induction of GM-CSF gene expression more clearly, we prepared and analyzed deletion mutants of GM-CSF promoter recombinant constructs. The results localized inducible expression to a 90-base-pair region (-53 to +37) which is active in uninfected and human T-cell leukemia virus-infected T-cell lines but not in resting or mitogen-stimulated B cells. DNase I footprinting experiments revealed protection of sequences contained within this region, including a repeated nucleotide sequence, CATT(A/T), which could serve as a core recognition sequence for a cellular transcription factor. Upstream of these GM-CSF promoter sequences is a 15-base-pair region (-193 to -179) which has negative regulatory activity in human T-cell leukemia virus-infected T cells. These studies revealed a complex pattern of regulation of GM-CSF expression in T cells; positive and negative regulatory sequences may play critical roles in controlling the expression of this potent granulopoietin in the bone marrow microenvironment and in localized inflammatory responses.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-181845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-2578615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-265521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-2984574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-2990035,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-3013622,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-3020435,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-3024757,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-3024966,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-3026643,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-3030566,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-3034432,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-3040054,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-3083507,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-3095427,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-3102073,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-312818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-3349904,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-3484427,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-3484428,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-3486886,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-3488777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-3488815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-3489188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-3489530,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-3490669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-3497344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-3500414,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-3528288,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-3561489,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-3708688,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-3876930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-3876931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-3877981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-3923623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-4041012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-6180322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-6322188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-6390681,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-6429853,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2838738-6960240
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
8
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1979-84
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2838738-B-Lymphocytes,
pubmed-meshheading:2838738-Base Sequence,
pubmed-meshheading:2838738-Cell Line,
pubmed-meshheading:2838738-Colony-Stimulating Factors,
pubmed-meshheading:2838738-Deltaretrovirus,
pubmed-meshheading:2838738-Deoxyribonuclease I,
pubmed-meshheading:2838738-Gene Expression Regulation,
pubmed-meshheading:2838738-Humans,
pubmed-meshheading:2838738-Molecular Sequence Data,
pubmed-meshheading:2838738-Nucleotide Mapping,
pubmed-meshheading:2838738-Promoter Regions, Genetic,
pubmed-meshheading:2838738-Recombinant Fusion Proteins,
pubmed-meshheading:2838738-T-Lymphocytes
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pubmed:year |
1988
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pubmed:articleTitle |
Characterization of the human granulocyte-macrophage colony-stimulating factor promoter region by genetic analysis: correlation with DNase I footprinting.
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pubmed:affiliation |
Division of Hematology-Oncology, UCLA School of Medicine 90024.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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