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rdf:type |
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lifeskim:mentions |
|
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pubmed:issue |
2
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pubmed:dateCreated |
1988-8-8
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pubmed:abstractText |
The specific binding of [3H]retinoids to cellular retinoid-binding proteins was measured directly by the cold acetone filtration method. After incubation of purified cellular retinoid-binding proteins with [3H]retinoids with or without competitors for 2-4 h, bound ligands were separated from free by filtration using cold acetone. Nonspecific binding of the ligands was reduced sufficiently to allow measurement of specific binding of [3H]retinoids to cellular retinoid-binding proteins. This method has the advantages of being rapid and practical and giving reproducible results.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0014-5793
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
233
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
255-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2838330-Adrenal Glands,
pubmed-meshheading:2838330-Animals,
pubmed-meshheading:2838330-Carrier Proteins,
pubmed-meshheading:2838330-Cattle,
pubmed-meshheading:2838330-Kinetics,
pubmed-meshheading:2838330-Receptors, Retinoic Acid,
pubmed-meshheading:2838330-Retinol-Binding Proteins,
pubmed-meshheading:2838330-Substrate Specificity,
pubmed-meshheading:2838330-Tretinoin,
pubmed-meshheading:2838330-Tritium,
pubmed-meshheading:2838330-Vitamin A
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pubmed:year |
1988
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pubmed:articleTitle |
Specific binding of [3H]retinoids to cellular retinoid-binding proteins.
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pubmed:affiliation |
National Cancer Center Research Institute, Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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