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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1988-7-29
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pubmed:databankReference |
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pubmed:abstractText |
The factor-dependent myeloid precursor cell line D35 mutates spontaneously at a frequency greater than 2.4 x 10(-7) to growth factor autonomy. This frequency could be increased at least 20-fold by retrovirus insertional mutagenesis. The isolation and characterization of factor-independent mutants allowed the identification of genes involved in growth autonomy. Mutants could be subdivided into two sets: those that secreted a stimulating factor (10/11) and those that did not (1/11). In one case, the factor released was distinct from previously characterized growth factors. In most mutants (6/9), the activation of a growth factor gene was associated with rearrangement that could be attributed to the insertion of a transposable-like element either 5' or 3' of the factor coding region in all cases examined, excluding oncogene involvement. All factor-independent mutants were tumorigenic, consistent with the hypothesis that growth-factor independence initiated by aberrant growth factor gene activation is an important and early step in tumorigenesis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
17
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pubmed:volume |
53
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
869-79
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2838175-Alleles,
pubmed-meshheading:2838175-Amino Acid Sequence,
pubmed-meshheading:2838175-Animals,
pubmed-meshheading:2838175-Base Sequence,
pubmed-meshheading:2838175-Cell Division,
pubmed-meshheading:2838175-Cell Line, Transformed,
pubmed-meshheading:2838175-Colony-Stimulating Factors,
pubmed-meshheading:2838175-DNA,
pubmed-meshheading:2838175-DNA Transposable Elements,
pubmed-meshheading:2838175-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:2838175-Growth Substances,
pubmed-meshheading:2838175-Hematopoietic Stem Cells,
pubmed-meshheading:2838175-Molecular Sequence Data,
pubmed-meshheading:2838175-Mutation,
pubmed-meshheading:2838175-Nucleic Acid Hybridization,
pubmed-meshheading:2838175-Oncogenes,
pubmed-meshheading:2838175-RNA, Messenger,
pubmed-meshheading:2838175-Sequence Homology, Nucleic Acid,
pubmed-meshheading:2838175-Transcription, Genetic
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pubmed:year |
1988
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pubmed:articleTitle |
Identification of genes involved in growth autonomy of hematopoietic cells by analysis of factor-independent mutants.
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pubmed:affiliation |
Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie an der Universität Hamburg, Federal Republic of Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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