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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
1988-7-28
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pubmed:abstractText |
Bovid herpesvirus-4 (BHV-4) isolates V.Test and LVR140, isolated from genital disease, respectively, in bull and in cow, and the reference strains Movar 33/63 and DN599 were compared by several methods: cross-serological relationship studied by indirect immunofluorescence; kinetics of intracellular and extracellular viral production; comparison of the mean plaque size; restriction analysis of viral DNA with restriction enzymes EcoRI, BamHI and HindIII. BHV-4 strains were serologically identical and the kinetics of viral production were very similar. Comparison of the mean plaque size allowed classification into 3 classes (Class I, Movar33/63; Class II, LVR140; Class III, V.Test and DN599) and restriction analysis of viral DNA revealed clear differences between the electrophoretic patterns of the four BHV-4 strains. The differentiation between BHV-4 strains can therefore be achieved by a biological method (mean plaque size) and by restriction analysis. The two genital isolates are easily differentiated by the two methods.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0378-1135
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
339-49
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:2837868-Analysis of Variance,
pubmed-meshheading:2837868-Animals,
pubmed-meshheading:2837868-Antibodies, Viral,
pubmed-meshheading:2837868-Cattle,
pubmed-meshheading:2837868-Cross Reactions,
pubmed-meshheading:2837868-DNA, Viral,
pubmed-meshheading:2837868-DNA Restriction Enzymes,
pubmed-meshheading:2837868-Electrophoresis, Agar Gel,
pubmed-meshheading:2837868-Female,
pubmed-meshheading:2837868-Fluorescent Antibody Technique,
pubmed-meshheading:2837868-Herpesviridae,
pubmed-meshheading:2837868-Kinetics,
pubmed-meshheading:2837868-Male,
pubmed-meshheading:2837868-Rabbits,
pubmed-meshheading:2837868-Viral Plaque Assay
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pubmed:year |
1988
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pubmed:articleTitle |
Biological and biochemical comparison of bovid herpesvirus-4 strains.
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pubmed:affiliation |
Department of Virology-Immunology, Faculty of Veterinary Medicine, University of Liège, Brussels, Belgium.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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