Linked Life Data

2837114

Source:http://linkedlifedata.com/resource/pubmed/id/2837114

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1988-7-5
pubmed:abstractText
The effectiveness of 1% saponin at 55 C (SAP), glutaraldehyde-borohydride-saponin (GBS), and modified GBS (MGBS) as fixatives for preserving cellular morphologic features, as well as antigenicity of intracellular and membrane-bound viral proteins of canine parainfluenza virus (CPIV) and canine distemper virus (CDV) was studied. Use of the same fixatives for light and electron microscopic immunocytochemical examination also was investigated. By light microscopy, CDV inclusions were readily detected after SAP and MGBS fixation, but not after GBS fixation; CPIV inclusions were detected after GBS and MGBS fixation, but not after SAP fixation. Ultrastructurally, SAP-treated cells had moderate to severe cytoplasmic artifacts, although CDV-associated cytoplasmic and membrane viral antigens were readily labeled. The CPIV-infected cells contained only a few positively labeled membrane-associated antigens and cytoplasmic nucleocapsids (NC). Although GBS-treated cells had excellent ultrastructural preservation, immunolabeling was unsatisfactory; CPIV-NC were labeled incompletely, and CDV-NC were unlabeled. After fixation with MGBS, immunolabeling of NC and membrane-associated viral proteins for both viruses was achieved, and the architecture of infected cells was preserved.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0002-9645
pubmed:author
pubmed:issnType
Print
pubmed:volume
49
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
477-81
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Fixation procedures for retention of cellular morphologic features and for preservation of immunoreactivity of canine paramyxovirus antigens.
pubmed:affiliation
Department of Veterinary Pathobiology, College of Veterinary Medicine, Ohio State University, Columbus 43210.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't