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Predicate | Object |
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rdf:type | |
lifeskim:mentions |
umls-concept:C0001443,
umls-concept:C0001455,
umls-concept:C0033640,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0035696,
umls-concept:C0036770,
umls-concept:C0205369,
umls-concept:C0439596,
umls-concept:C0443199,
umls-concept:C0851285,
umls-concept:C0851827,
umls-concept:C1701901,
umls-concept:C1711351
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pubmed:issue |
6
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pubmed:dateCreated |
1988-6-28
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pubmed:abstractText |
In the present study we have examined the effects of FSH, forskolin, and (Bu)2cAMP on messenger RNA (mRNA) levels for all known subunits of cAMP-dependent protein kinase in rat Sertoli cells, using newly developed complementary DNA (cDNA) probes. mRNAs for the three regulatory subunits [RI alpha, RII51, (RII beta), and RII54 (RII alpha)] and the catalytic subunit C alpha were shown to be present in cultured rat Sertoli cells, whereas mRNAs for the subunits designated RI beta and C beta were below the level of detection. A high-levelled, concentration-dependent increase in a 3.2 kilobase mRNA for RII51 was observed when cultured immature Sertoli cells were incubated with increasing concentrations of (Bu)2cAMP (10(-6) to 5 X 10(-3) M) for 16 h. Densitometric scanning indicated a maximal stimulation by (Bu)2cAMP of 30- to 40-fold. Incubation with forskolin (100 microM) and FSH (200 ng/ml) gave rise to a smaller but significant increase in mRNA for RII51. When cultured Sertoli cells were incubated in the presence of 10(-4) M (Bu)2cAMP for varying time periods, there was a biphasic regulation of mRNA for RII51. (Bu)2cAMP caused an initial increase in mRNA for RII51 with maximal levels obtained after 10-16 h, after which a time-dependent decrease was observed. For the other three subunits present in Sertoli cells (RI alpha, RII54, and C alpha) a smaller but significant stimulation by (Bu)2cAMP and forskolin (2-4 fold) was seen. The functional implications of these changes in mRNA levels for the different subunits of cAMP-dependent protein kinase have not yet been revealed. However, our data clearly demonstrate differential regulation of the various subunits of cAMP-dependent protein kinase in Sertoli cells. Furthermore, these results document the presence of distinct adaptational changes taking place at the level of cAMP-dependent protein kinase in response to long term elevation of cAMP.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bucladesine,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Follicle Stimulating Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Forskolin,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0013-7227
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
122
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2658-66
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:2836170-Animals,
pubmed-meshheading:2836170-Bucladesine,
pubmed-meshheading:2836170-Cells, Cultured,
pubmed-meshheading:2836170-Cyclic AMP,
pubmed-meshheading:2836170-DNA,
pubmed-meshheading:2836170-Follicle Stimulating Hormone,
pubmed-meshheading:2836170-Forskolin,
pubmed-meshheading:2836170-Gene Expression Regulation,
pubmed-meshheading:2836170-Kinetics,
pubmed-meshheading:2836170-Male,
pubmed-meshheading:2836170-Protein Kinases,
pubmed-meshheading:2836170-RNA, Messenger,
pubmed-meshheading:2836170-Rats,
pubmed-meshheading:2836170-Rats, Inbred Strains,
pubmed-meshheading:2836170-Sertoli Cells
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pubmed:year |
1988
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pubmed:articleTitle |
Differential regulation of messenger ribonucleic acids for specific subunits of cyclic adenosine 3',5'-monophosphate (cAMP)-dependent protein kinase by cAMP in rat Sertoli cells.
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pubmed:affiliation |
Institute of Pathology, Rikshospitalet, Oslo, Norway.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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