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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1988-6-14
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pubmed:abstractText |
Interactions between integral proteins of the plasma membrane and the cytoskeleton may be important for localizing certain membrane proteins in a nonrandom fashion at specialized domains of the cell surface. Here, we show that ankyrin, the key protein for the linkage of the erythrocyte anion exchanger (band 3) to the spectrin-based membrane cytoskeleton, is also present in kidney distal tubular cells where ankyrin is precisely colocalized with Na+,K+-ATPase. Both proteins are confined to the basolateral plasma membrane and are absent from the apical membrane, the junctional complex and the membrane surface that contacts the basal lamina. Purified Na+,K+-ATPase of sheep and pig kidney contains a binding site for erythrocyte ankyrin as demonstrated by immunoprecipitation experiments. A band 3-like binding site for ankyrin is likely, since binding of ankyrin to Na+,K+-ATPase could be inhibited in a competitive fashion by the isolated cytoplasmic domain of erythrocyte band 3.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0171-9335
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
45
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
230-7
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:2835237-Animals,
pubmed-meshheading:2835237-Ankyrins,
pubmed-meshheading:2835237-Blood Proteins,
pubmed-meshheading:2835237-Cell Membrane,
pubmed-meshheading:2835237-Chemical Precipitation,
pubmed-meshheading:2835237-Epithelium,
pubmed-meshheading:2835237-Kidney Tubules,
pubmed-meshheading:2835237-Kidney Tubules, Distal,
pubmed-meshheading:2835237-Membrane Proteins,
pubmed-meshheading:2835237-Microscopy, Electron,
pubmed-meshheading:2835237-Rats,
pubmed-meshheading:2835237-Sodium-Potassium-Exchanging ATPase
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pubmed:year |
1988
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pubmed:articleTitle |
Colocalization and coprecipitation of ankyrin and Na+,K+-ATPase in kidney epithelial cells.
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pubmed:affiliation |
Department of Anatomy and Cell Biology, University of Marburg/Federal Republic of Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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