Linked Life Data

2833564

Source:http://linkedlifedata.com/resource/pubmed/id/2833564

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1988-5-24
pubmed:abstractText
The reactions of nitrite and oxygen with the cytochrome d oxidase of Escherichia coli were studied, following growth of cells on glycerol with fumarate as respiratory oxidant. Optical difference spectroscopy was used to investigate the kinetics of product formation during the reaction of the respiratory chain with nitrite. Two kinetically distinct species were formed in the reaction with nitrite; these had spectral features at 438 nm and 630 nm. These observations indicate that the cytochrome d does not contribute significantly to absorbance in the Soret region, and that changes elicited by ligand binding in the Soret region are largely attributable to haemoprotein b-590. Inhibition of respiratory oxidase activity by nitrite was also investigated. The inhibition was competitive with oxygen (Ki 0.83 mM, pH 7), which allowed analysis of the reaction of the oxidase with oxygen itself. The reaction with oxygen was cooperative with an apparent number of oxygen-binding sites, n, of 1.26 at pH 7, increasing to 1.72 at pH 6. We propose a model for the oxidase in which there are two ligand-binding sites.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-1287
pubmed:author
pubmed:issnType
Print
pubmed:volume
133
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3247-55
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
The respiratory chain of anaerobically grown Escherichia coli: reactions with nitrite and oxygen.
pubmed:affiliation
Department of Biochemistry and Microbiology, University of St Andrews, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't