Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12
|
| pubmed:dateCreated |
1988-5-18
|
| pubmed:abstractText |
Two monoclonal anti-idiotypic antibodies (anti-Id-135 and anti-Id-14, both of the IgM class) which interact with the binding site of opioid receptors were generated. A monoclonal anti-beta-endorphin antibody (3-E7) which displays binding characteristics for opioid ligands similar to opioid receptors served as the antigen (Gramsch, C., Meo, T., Riethmüller, G., and Herz, A., (1983) J. Neurochem. 40, 1220-1226; Meo, T., Gramsch, C., Inan, R., Höllt, V., Weber, E., Herz, A., and Riethmüller, G. (1983) Proc. Natl. Acad. Sci. U.S.A. 80, 4048-4088) and the hybridomas obtained were screened for anti-idiotypic antibodies with Fab fragments of 3-E7. The anti-idiotypes were then screened for opioid binding to rat brain membrane receptors, yielding several positive clones two of which were more intensively studied. Both anti-idiotypic antibodies were about equally potent in displacing the mu- and delta-opioid receptor ligands [3H]dihydromorphine, 125I-labeled beta-endorphin, [D-Ala2, D-Leu5-3H]enkephalin and [3H]naloxone from rat brain membrane opioid receptors; no interaction was observed with the kappa-ligands [3H]ethylketazocine or [3H]bremazocine. The anti-idiotypic antibodies were able to precipitate [3H] diprenorphine binding sites from solubilized opioid receptor preparations. In addition, both antibodies showed opioid antagonistic properties as demonstrated by their abilities to block the inhibitory effect of [D-Ala2, D-Leu5-3H]enkephalin on prostaglandin E1-stimulated cAMP accumulation in NG 108-15 hybrid cells. Our findings demonstrate the successful generation of monoclonal antibodies interacting with membrane-bound and solubilized opioid receptors of the mu- and delta-type.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Dihydromorphine,
http://linkedlifedata.com/resource/pubmed/chemical/Enkephalin, Leucine,
http://linkedlifedata.com/resource/pubmed/chemical/Enkephalin, Leucine-2-Alanine,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Idiotypes,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin M,
http://linkedlifedata.com/resource/pubmed/chemical/Naloxone,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Opioid,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Endorphin
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
25
|
| pubmed:volume |
263
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
5853-9
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:2833518-Animals,
pubmed-meshheading:2833518-Antibodies, Monoclonal,
pubmed-meshheading:2833518-Antigens,
pubmed-meshheading:2833518-Brain,
pubmed-meshheading:2833518-Cell Membrane,
pubmed-meshheading:2833518-Cyclic AMP,
pubmed-meshheading:2833518-Dihydromorphine,
pubmed-meshheading:2833518-Enkephalin, Leucine,
pubmed-meshheading:2833518-Enkephalin, Leucine-2-Alanine,
pubmed-meshheading:2833518-Hybridomas,
pubmed-meshheading:2833518-Immunoglobulin G,
pubmed-meshheading:2833518-Immunoglobulin Idiotypes,
pubmed-meshheading:2833518-Immunoglobulin M,
pubmed-meshheading:2833518-Immunosorbent Techniques,
pubmed-meshheading:2833518-Mice,
pubmed-meshheading:2833518-Mice, Inbred BALB C,
pubmed-meshheading:2833518-Naloxone,
pubmed-meshheading:2833518-Rats,
pubmed-meshheading:2833518-Receptors, Opioid,
pubmed-meshheading:2833518-Tumor Cells, Cultured,
pubmed-meshheading:2833518-beta-Endorphin
|
| pubmed:year |
1988
|
| pubmed:articleTitle |
Monoclonal anti-idiotypic antibodies to opioid receptors.
|
| pubmed:affiliation |
Department of Neuropharmacology, Max-Planck-Institut für Psychiatrie, Planegg-Martinsried, Federal Republic of Germany.
|
| pubmed:publicationType |
Journal Article
|