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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
1988-5-18
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pubmed:databankReference |
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pubmed:abstractText |
Amphibian skin is well known as a source of peptides homologous to bioactive peptides found in mammalian gut and brain. A systematic investigation of the skin secretions from Xenopus laevis revealed several peptides not derivable from known precursors. The sequence elucidation, utilizing fast atom bombardment/mass spectrometry, of two peptides, PGS and PGS Gly-10;Lys-22, is reported. These have been independently characterized and named magainins and found to have antimicrobial activity. A mixed sequence oligonucleotide probe complementary to the mRNA sequence coding for PGS was synthesized and used to screen a Xenopus skin cDNA library. A full length cDNA species encoding prepro-PGS was isolated and characterized, and its sequence is reported. The deduced precursor sequence was found to contain one copy of PGS Gly-10;Lys-22 and five copies of PGS. The proteolytic processing of this prepro-polypeptide is discussed.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antimicrobial Cationic Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Restriction Enzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Magainins,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Precursors,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Xenopus Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/magainin 1 peptide, Xenopus,
http://linkedlifedata.com/resource/pubmed/chemical/magainin 2 peptide, Xenopus
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
263
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5745-51
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:2833514-Amino Acid Sequence,
pubmed-meshheading:2833514-Animals,
pubmed-meshheading:2833514-Antimicrobial Cationic Peptides,
pubmed-meshheading:2833514-Base Sequence,
pubmed-meshheading:2833514-Chromatography, High Pressure Liquid,
pubmed-meshheading:2833514-DNA,
pubmed-meshheading:2833514-DNA Restriction Enzymes,
pubmed-meshheading:2833514-Magainins,
pubmed-meshheading:2833514-Male,
pubmed-meshheading:2833514-Mass Spectrometry,
pubmed-meshheading:2833514-Molecular Sequence Data,
pubmed-meshheading:2833514-Peptides,
pubmed-meshheading:2833514-Protein Precursors,
pubmed-meshheading:2833514-RNA, Messenger,
pubmed-meshheading:2833514-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:2833514-Skin,
pubmed-meshheading:2833514-Xenopus Proteins,
pubmed-meshheading:2833514-Xenopus laevis
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pubmed:year |
1988
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pubmed:articleTitle |
The cDNA sequence coding for prepro-PGS (prepro-magainins) and aspects of the processing of this prepro-polypeptide.
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pubmed:affiliation |
University Chemical Laboratories, Cambridge, United Kingdom.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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