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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1988-5-12
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pubmed:abstractText |
We report the cAMP-dependent phosphorylation of the chemotactic receptor of Dictyostelium discoideum in partially purified plasma membranes. The protein kinase responsible for receptor phosphorylation is associated with this fraction and preferentially phosphorylates the ligand-occupied form of the receptor. 8-Azido[32P]cAMP labeling of the cell surface has shown that the cAMP receptor exists in two forms. A 45-kDa protein is predominant on unstimulated cells. cAMP stimulation results in an increased receptor phosphorylation such that the receptor migrates on NaDodSO4/PAGE as a 47-kDa protein. Phosphorylation of the chemotactic receptor is not detected in membrane preparations unless cAMP is added to the incubation mixture. Only under those conditions is the phosphorylated 47-kDa form observed. The requirement for cAMP reflects the fact that the kinase involved preferentially uses the ligand-occupied receptor as a substrate. In vitro phosphorylation of the receptor does not involve tyrosine residues. The enzyme does not appear to be a cAMP- or cGMP-dependent protein kinase nor is it sensitive to guanine nucleotides, Ca2+/calmodulin, Ca2+/phospholipid, or EGTA. Similarities with the beta-adrenergic receptor protein kinase are discussed.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832847-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832847-16453431,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832847-171079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832847-189939,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832847-2871555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832847-2880607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832847-2982884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832847-3014512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832847-3030559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832847-3036840,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832847-3110144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832847-3122750,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832847-38740,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832847-3998231,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832847-4631665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832847-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832847-5530748,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832847-6100962,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832847-6249827,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832847-6256362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832847-6263735,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832847-6268062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832847-6288711,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832847-6309778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832847-6894148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832847-7408904
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
85
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2181-5
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pubmed:dateRevised |
2010-9-9
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pubmed:meshHeading |
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pubmed:year |
1988
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pubmed:articleTitle |
An unusual protein kinase phosphorylates the chemotactic receptor of Dictyostelium discoideum.
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pubmed:affiliation |
Edward A. Doisey Department of Biochemistry, St. Louis University School of Medicine, MO 63104.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
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