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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1988-5-12
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pubmed:abstractText |
Mutations that define the spoIIE locus of Bacillus subtilis block sporulation at an early stage and recently were shown to prevent the proteolytic processing of sigma E (sigma 29) into its active form, an event that is believed to control critical changes in gene expression during the second hour of development. By taking advantage of two Tn917-mediated insertional mutations in spoIIE, we have cloned DNA spanning the locus. Gene disruption experiments with subcloned fragments transferred to integrational vectors revealed that the locus consisted of a single transcription unit about 2.5 kilobase pairs in size. Transcriptional lacZ fusions were used to show that expression of this transcription unit initiated at 1.5 h after the end of log-phase growth and depended upon the products of all spo0 loci. Expression was directed by a single promoter whose position was determined by high-resolution S1 protection mapping. A deletion analysis of the promoter region was also carried out, with novel integrational vectors based on derivatives of coliphage M13. The results indicated that a region of DNA extending from 183 to 118 base pairs upstream from the start point of transcription was required for full activity of the spoIIE promoter. The presumptive RNA polymerase-binding region of the promoter exhibited striking similarity to the spoIIG promoter and featured perfect but unusually spaced -10 and -35 consensus sequences for sigma A (sigma 43)-associated RNA polymerase.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832371-1008685,
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
170
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1598-609
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2832371-Bacillus subtilis,
pubmed-meshheading:2832371-Base Sequence,
pubmed-meshheading:2832371-Chromosome Deletion,
pubmed-meshheading:2832371-Cloning, Molecular,
pubmed-meshheading:2832371-DNA, Bacterial,
pubmed-meshheading:2832371-DNA Transposable Elements,
pubmed-meshheading:2832371-DNA-Directed RNA Polymerases,
pubmed-meshheading:2832371-Gene Expression Regulation,
pubmed-meshheading:2832371-Genes, Bacterial,
pubmed-meshheading:2832371-Molecular Sequence Data,
pubmed-meshheading:2832371-Mutation,
pubmed-meshheading:2832371-Operon,
pubmed-meshheading:2832371-Promoter Regions, Genetic,
pubmed-meshheading:2832371-Spores, Bacterial,
pubmed-meshheading:2832371-Transcription, Genetic,
pubmed-meshheading:2832371-beta-Galactosidase
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pubmed:year |
1988
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pubmed:articleTitle |
Characterization of the promoter region of the Bacillus subtilis spoIIE operon.
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pubmed:affiliation |
Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia 19104.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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