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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
13
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pubmed:dateCreated |
1988-5-4
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pubmed:abstractText |
Mutation of the alpha-actinin gene in Dictyostelium has been achieved by transforming cells with the Dictyostelium transformation vector pDNeoII containing a 1.2 kb fragment of the alpha-actinin gene. Transformants deficient in alpha-actinin, an actin-binding protein, produced an altered mRNA that lacked the 3' portion of the coding region. The defect in alpha-actinin production was not due to integration of the vector within the gene, but was apparently caused by errors produced during homologous recombination between the introduced alpha-actinin sequence and its complementary sequence in the coding region of the endogenous gene.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832153-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832153-16453689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832153-2420519,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832153-2434948,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832153-2991530,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832153-3002636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832153-3025622,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832153-3037345,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832153-347451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832153-3576221,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832153-3576222,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832153-3622778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832153-3745276,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832153-3785372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832153-3818609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832153-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832153-3956480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832153-4075402,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832153-4092928,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832153-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832153-6098825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832153-6099327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832153-6273866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2832153-6327743
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
6
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4143-8
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pubmed:dateRevised |
2010-9-9
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pubmed:meshHeading |
pubmed-meshheading:2832153-Actinin,
pubmed-meshheading:2832153-DNA Restriction Enzymes,
pubmed-meshheading:2832153-Dictyostelium,
pubmed-meshheading:2832153-Genes,
pubmed-meshheading:2832153-Genes, Fungal,
pubmed-meshheading:2832153-Genetic Vectors,
pubmed-meshheading:2832153-RNA, Messenger,
pubmed-meshheading:2832153-Recombination, Genetic,
pubmed-meshheading:2832153-Transformation, Genetic
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pubmed:year |
1987
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pubmed:articleTitle |
Homologous recombination in the Dictyostelium alpha-actinin gene leads to an altered mRNA and lack of the protein.
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pubmed:affiliation |
Max-Planck-Institut für Biochemie, Martinsried, FRG.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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