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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1988-4-19
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pubmed:abstractText |
Transforming growth factor-beta (TGF-beta) increases the steady-state RNA levels of several fibroblast extracellular matrix proteins. Using DNA transfection, we show that TGF-beta stimulates the activity of the mouse alpha 2(l) collagen promoter 5- to 10-fold in mouse NIH 3T3 and rat osteosarcoma cells. Deletion analysis indicates that a segment of this promoter between -350 and -300, overlapping a nuclear factor 1 (NF1) binding site, is needed for TGF-beta stimulation. A 3 bp substitution mutation abolishing NF1 binding to this site inhibits TGF-beta activation. Insertion of this NF1 binding site 5' to the SV40 early promoter makes the promoter TGF-beta inducible, but the 3 bp substitution does not. Similarly, when the NF1 binding site at the replication origin of adenovirus 2 and 5 is inserted 5' to the SV40 promoter, the promoter responds to TGF-beta. Therefore an NF1 binding site mediates the transcriptional activation of the mouse alpha 2(l) collagen promoter by TGF-beta.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CCAAT-Enhancer-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Collagen,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Substances,
http://linkedlifedata.com/resource/pubmed/chemical/NFI Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Y-Box-Binding Protein 1,
http://linkedlifedata.com/resource/pubmed/chemical/YBX1 protein, human
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
12
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pubmed:volume |
52
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
405-14
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:2830985-Animals,
pubmed-meshheading:2830985-Base Sequence,
pubmed-meshheading:2830985-Binding Sites,
pubmed-meshheading:2830985-CCAAT-Enhancer-Binding Proteins,
pubmed-meshheading:2830985-Collagen,
pubmed-meshheading:2830985-DNA-Binding Proteins,
pubmed-meshheading:2830985-Fibroblasts,
pubmed-meshheading:2830985-Growth Substances,
pubmed-meshheading:2830985-Mice,
pubmed-meshheading:2830985-Molecular Sequence Data,
pubmed-meshheading:2830985-Mutation,
pubmed-meshheading:2830985-NFI Transcription Factors,
pubmed-meshheading:2830985-Nuclear Proteins,
pubmed-meshheading:2830985-Peptides,
pubmed-meshheading:2830985-Promoter Regions, Genetic,
pubmed-meshheading:2830985-Rats,
pubmed-meshheading:2830985-Simian virus 40,
pubmed-meshheading:2830985-Transcription Factors,
pubmed-meshheading:2830985-Transfection,
pubmed-meshheading:2830985-Transforming Growth Factors,
pubmed-meshheading:2830985-Y-Box-Binding Protein 1
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pubmed:year |
1988
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pubmed:articleTitle |
A nuclear factor 1 binding site mediates the transcriptional activation of a type I collagen promoter by transforming growth factor-beta.
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pubmed:affiliation |
Laboratory of Molecular Biology, National Cancer Institute, Bethesda, Maryland 20892.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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