Linked Life Data

2828982

Source:http://linkedlifedata.com/resource/pubmed/id/2828982

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1988-3-18
pubmed:abstractText
Biotinylated derivatives of beta h-endorphin (beta h-EP) with C6 spacer arm, inserted between biotin and beta h-EP, were synthesized and isolated by HPLC. Liquid secondary ion mass spectrometry (LSIMS) indicated the presence of 1 to 4 biotin substituents per beta h-EP molecule, and in combination with the analysis of tryptic peptide fragments, specified the location of the biotinylated lysine residue. Affinities to mu receptors decreased with increasing biotinylation number. Association of the biotinylated ligands with avidin retained or even enhanced IC50 values at the mu site, thus, matching the relative binding affinity of underivatized beta h-EP with the monobiotinylated derivatives. Hence, monobiotinylated beta h-EP represents a versatile opioid receptor probe.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1046-9516
pubmed:author
pubmed:issnType
Print
pubmed:volume
75
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
33-6
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Development of a biotin-avidin probe for detecting opioid receptors.
pubmed:affiliation
Department of Pharmacy and Pharmaceutical Chemistry, University of California, San Francisco 94143.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.