rdf:type |
|
lifeskim:mentions |
umls-concept:C0015252,
umls-concept:C0018284,
umls-concept:C0032143,
umls-concept:C0439590,
umls-concept:C0449297,
umls-concept:C0596988,
umls-concept:C0728940,
umls-concept:C1280500,
umls-concept:C1514562,
umls-concept:C1515655,
umls-concept:C1880389,
umls-concept:C1883204,
umls-concept:C1883221
|
pubmed:issue |
4
|
pubmed:dateCreated |
1988-3-7
|
pubmed:abstractText |
The complete cDNA for human tissue-type plasminogen activator (t-PA) was cloned and sequenced. A mutant was constructed by using in vitro site-specific mutagenesis to delete the region encoding the growth factor domain (amino acids 51-87 inclusive). Normal and mutant t-PA species were produced using two mammalian expression systems (in human HeLa cells and mouse C127 cells). The clearance of mutant and normal t-PA from plasma was examined in vivo using a guinea pig model. Mutant t-PA derived from HeLa or C127 cells was cleared much more slowly than the cognate normal t-PA. The potential role of the growth factor domain in the recognition of t-PA by the hepatic clearance mechanism is discussed.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Feb
|
pubmed:issn |
0021-9258
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:day |
5
|
pubmed:volume |
263
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
1599-602
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:2828346-Animals,
pubmed-meshheading:2828346-Bacterial Proteins,
pubmed-meshheading:2828346-Base Sequence,
pubmed-meshheading:2828346-Cloning, Molecular,
pubmed-meshheading:2828346-DNA,
pubmed-meshheading:2828346-DNA Restriction Enzymes,
pubmed-meshheading:2828346-Deoxyribonucleases, Type II Site-Specific,
pubmed-meshheading:2828346-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2828346-Gene Expression Regulation,
pubmed-meshheading:2828346-Guinea Pigs,
pubmed-meshheading:2828346-Humans,
pubmed-meshheading:2828346-Mutation,
pubmed-meshheading:2828346-Structure-Activity Relationship,
pubmed-meshheading:2828346-Tissue Plasminogen Activator
|
pubmed:year |
1988
|
pubmed:articleTitle |
A tissue-type plasminogen activator mutant with prolonged clearance in vivo. Effect of removal of the growth factor domain.
|
pubmed:affiliation |
Beecham Pharmaceuticals Research Division, Biosciences Research Centre, Epsom, Surrey, United Kingdom.
|
pubmed:publicationType |
Journal Article
|