Linked Life Data

282758

Source:http://linkedlifedata.com/resource/pubmed/id/282758

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1979-3-24
pubmed:abstractText
Data were obtained concerning the usefulness of discontinuous Ficoll and Isopaque-Ficoll gradients in separating malignant from non-malignant cells from a variety of sources. Most of the material was from effusions, but there is no theoretical restriction of the methods to effusions. Animal and human material were studied, utilizing non-malignant as well as malignant material, the former acting as controls. In the discontinuous Ficoll gradient, concentration of malignant cells at a specific interface was mainly a function of cell density. Thus, while most malignant cells banded in the last two fractions, adenocarcinomas, especially those high in mucus (which decreases total cell density) banded higher in the gradient. The Isopaque-Ficoll technique also proved useful in concentrating malignant cells. The advantage of the discontinuous Ficoll gradient was its ability to separate cells from small specimens (1-10 X 10(6) cells). Contrarily, the Isopaque-Ficoll gradient was preferable for processing specimens of larger volumes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
0001-5547
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
584-8
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:articleTitle
Gradient separation of normal and malignant cells. II. Application to in vivo tumor diagnosis.
pubmed:publicationType
Journal Article