Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1988-2-9
pubmed:abstractText
Neisseria gonorrhoeae strains WR302 and PGH3-2 were characterized with respect to their restriction-modification phenotype. WR302 DNA was cleaved by HaeIII, indicating the lack of methylation at the GGCC sequence. PGH3-2 produced NgoSI (an isoschizomer of NgoII). WR302 produced a restriction enzyme with a recognition sequence different from that of NgoI, NgoII, or NgoIII. Plasmid pFT180 isolated from WR302 was unable to transform PGH3-2, whereas plasmid pFT180 isolated from PGH3-2 was able to transform PGH3-2 at a very high frequency. When plasmid pFT180 isolated from WR302 was methylated in vitro with meth M. HaeIII, this plasmid was able to transform PGH3-2. NgoSI was able to restrict WR302 DNA in vitro, whereas it was incapable of restricting PGH3-2 DNA in vitro. When the self-transmissible R factor pFT6 was mobilized from WR302 to PGH3-2 by conjugation, a 1-order-of-magnitude difference in transfer frequencies was observed, as compared with an isogenic cross. The data indicate that host-mediated restriction can prevent the gonococcus from acquiring DNA via transformation but not via conjugation.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2826333-108261, http://linkedlifedata.com/resource/pubmed/commentcorrection/2826333-13888713, http://linkedlifedata.com/resource/pubmed/commentcorrection/2826333-14047217, http://linkedlifedata.com/resource/pubmed/commentcorrection/2826333-2987885, http://linkedlifedata.com/resource/pubmed/commentcorrection/2826333-3918988, http://linkedlifedata.com/resource/pubmed/commentcorrection/2826333-3934078, http://linkedlifedata.com/resource/pubmed/commentcorrection/2826333-418060, http://linkedlifedata.com/resource/pubmed/commentcorrection/2826333-4583233, http://linkedlifedata.com/resource/pubmed/commentcorrection/2826333-4958881, http://linkedlifedata.com/resource/pubmed/commentcorrection/2826333-6116723, http://linkedlifedata.com/resource/pubmed/commentcorrection/2826333-6257650, http://linkedlifedata.com/resource/pubmed/commentcorrection/2826333-6292160, http://linkedlifedata.com/resource/pubmed/commentcorrection/2826333-6304463, http://linkedlifedata.com/resource/pubmed/commentcorrection/2826333-6314109, http://linkedlifedata.com/resource/pubmed/commentcorrection/2826333-6319238, http://linkedlifedata.com/resource/pubmed/commentcorrection/2826333-6327618, http://linkedlifedata.com/resource/pubmed/commentcorrection/2826333-6411687, http://linkedlifedata.com/resource/pubmed/commentcorrection/2826333-6432693, http://linkedlifedata.com/resource/pubmed/commentcorrection/2826333-6780524, http://linkedlifedata.com/resource/pubmed/commentcorrection/2826333-97270
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0019-9567
pubmed:author
pubmed:issnType
Print
pubmed:volume
56
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
112-6
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Restriction of plasmid DNA during transformation but not conjugation in Neisseria gonorrhoeae.
pubmed:affiliation
Department of Microbiology, University of Maryland, College Park 20742.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't