|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0001455,
umls-concept:C0007634,
umls-concept:C0012472,
umls-concept:C0020731,
umls-concept:C0021554,
umls-concept:C0022646,
umls-concept:C0022675,
umls-concept:C0033268,
umls-concept:C0205312,
umls-concept:C0220781,
umls-concept:C0332291,
umls-concept:C0596233,
umls-concept:C1883254
|
|
pubmed:issue |
35
|
|
pubmed:dateCreated |
1988-1-21
|
|
pubmed:abstractText |
The effects of islet-activating protein (pertussis toxin) on bradykinin-mediated inositol trisphosphate labeling, prostaglandin E2 production, and calcium mobilization in rabbit renal papillary collecting tubule cells were assessed. Islet-activating protein induced time and concentration-dependent decreases in bradykinin-stimulated prostaglandin E2 production. Islet-activating protein induced increases in basal cyclic AMP levels but not in arginine vasopressin-stimulated cAMP. This effect could be inhibited by prior incubation with 2',5'-dideoxyadenosine, an inhibitor of adenylate cyclase. Although cAMP and cAMP analogues were able to inhibit both basal and bradykinin-stimulated prostaglandin E2 formation, the inhibitory effects of islet-activating protein on prostaglandin E2 formation and inositol trisphosphate labeling were observed in the presence of dideoxyadenosine. Moreover, islet-activating protein lowered both the basal and kinin-stimulated cytosolic calcium concentration as assessed by Quin 2 fluorescence. Finally, incubation of a membrane fraction of papillary cells with islet-activating protein resulted in the ADP-ribosylation of a 39/41-kDa doublet. These data support the role of a guanine nucleotide regulatory protein in bradykinin-mediated signal transduction in rabbit papillary collecting tubule cells.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2',5'-dideoxyadenosine,
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Diphosphate Ribose,
http://linkedlifedata.com/resource/pubmed/chemical/Adenylate Cyclase Toxin,
http://linkedlifedata.com/resource/pubmed/chemical/Aminoquinolines,
http://linkedlifedata.com/resource/pubmed/chemical/Arginine Vasopressin,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxyadenosines,
http://linkedlifedata.com/resource/pubmed/chemical/Dideoxyadenosine,
http://linkedlifedata.com/resource/pubmed/chemical/Dinoprostone,
http://linkedlifedata.com/resource/pubmed/chemical/Inositol Phosphates,
http://linkedlifedata.com/resource/pubmed/chemical/Kinins,
http://linkedlifedata.com/resource/pubmed/chemical/Pertussis Toxin,
http://linkedlifedata.com/resource/pubmed/chemical/Prostaglandins E,
http://linkedlifedata.com/resource/pubmed/chemical/Quin2,
http://linkedlifedata.com/resource/pubmed/chemical/Sugar Phosphates,
http://linkedlifedata.com/resource/pubmed/chemical/Virulence Factors, Bordetella
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Dec
|
|
pubmed:issn |
0021-9258
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
15
|
|
pubmed:volume |
262
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
17083-7
|
|
pubmed:dateRevised |
2007-11-14
|
|
pubmed:meshHeading |
pubmed-meshheading:2824514-Adenosine Diphosphate Ribose,
pubmed-meshheading:2824514-Adenylate Cyclase Toxin,
pubmed-meshheading:2824514-Aminoquinolines,
pubmed-meshheading:2824514-Animals,
pubmed-meshheading:2824514-Arginine Vasopressin,
pubmed-meshheading:2824514-Calcium,
pubmed-meshheading:2824514-Cyclic AMP,
pubmed-meshheading:2824514-Deoxyadenosines,
pubmed-meshheading:2824514-Dideoxyadenosine,
pubmed-meshheading:2824514-Dinoprostone,
pubmed-meshheading:2824514-Inositol Phosphates,
pubmed-meshheading:2824514-Kidney Tubules,
pubmed-meshheading:2824514-Kidney Tubules, Collecting,
pubmed-meshheading:2824514-Kinins,
pubmed-meshheading:2824514-Pertussis Toxin,
pubmed-meshheading:2824514-Prostaglandins E,
pubmed-meshheading:2824514-Rabbits,
pubmed-meshheading:2824514-Sugar Phosphates,
pubmed-meshheading:2824514-Virulence Factors, Bordetella
|
|
pubmed:year |
1987
|
|
pubmed:articleTitle |
Islet activating protein inhibits kinin-stimulated inositol phosphate production, calcium mobilization, and prostaglandin E2 synthesis in renal papillary collecting tubule cells independent of cyclic AMP.
|
|
pubmed:affiliation |
Department of Internal Medicine, University of Michigan Medical School, Ann Arbor.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
