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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
1988-1-14
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pubmed:abstractText |
Escherichia coli mutants conditionally defective in the conversion of pantothenate to coenzyme A were isolated and characterized. The gene was designated coaA and localized between argEH and rpoB near min 90 of the chromosome. The coaA15(Ts) mutation caused a temperature-sensitive growth phenotype and temperature-dependent inactivation of pantothenate kinase activity assayed both in vivo and in vitro. At 30 degrees C, coaA15(Ts) extracts contained less than 20% of the wild-type pantothenate kinase activity; the kinase had near normal kinetic constants for the substrates ATP and pantothenate and was inhibited by coenzyme A to the same degree as the wild-type enzyme. These data define the coaA gene as the structural gene for pantothenate kinase.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2824448-13278318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2824448-14320488,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2824448-230178,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2824448-2995137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2824448-2995306,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2824448-2999132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2824448-3014919,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2824448-3029083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2824448-3888959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2824448-4337331,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2824448-5074271,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2824448-5338813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2824448-6250539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2824448-6348505,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2824448-6370952,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2824448-6796563,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2824448-7037743,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2824448-7084227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2824448-7107640
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
169
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5795-800
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2824448-Coenzyme A,
pubmed-meshheading:2824448-Escherichia coli,
pubmed-meshheading:2824448-Genes,
pubmed-meshheading:2824448-Genes, Bacterial,
pubmed-meshheading:2824448-Mutation,
pubmed-meshheading:2824448-Pantothenic Acid,
pubmed-meshheading:2824448-Phenotype,
pubmed-meshheading:2824448-Phosphorylation,
pubmed-meshheading:2824448-Phosphotransferases,
pubmed-meshheading:2824448-Phosphotransferases (Alcohol Group Acceptor),
pubmed-meshheading:2824448-Temperature,
pubmed-meshheading:2824448-Transduction, Genetic,
pubmed-meshheading:2824448-beta-Alanine
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pubmed:year |
1987
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pubmed:articleTitle |
Isolation and characterization of temperature-sensitive pantothenate kinase (coaA) mutants of Escherichia coli.
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pubmed:affiliation |
Department of Biochemistry, St. Jude Children's Research Hospital, Memphis, Tennessee 38101.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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