Linked Life Data

2824289

Source:http://linkedlifedata.com/resource/pubmed/id/2824289

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1988-1-19
pubmed:abstractText
Using recombinant DNA techniques, the Tn10-specific tetA gene (coding for tetracycline resistance) has been mutagenized by insertion of a streptomycin-resistance or a kanamycin-resistance gene. The insertions occurred at loci separated by 920 bp. The mutated tetA fragments, respectively designated as Tes (for tetracycline-streptomycin) and Tek (for tetracycline-kanamycin), were subsequently cloned into a phage lambda cIII+cIts857cII+ in replacement of the att lambda region. The two recombinant phages are convenient delivery vehicles which permit the in vivo substitution of the tetA locus of any Tn10 insertion with the Tes or the Tek fragment. The procedure involves two selectable steps: (i) integration of a lambda-Tes (or lambda-Tek) prophage into the Tn10 of interest; (ii) excision of the prophage by a second exchange which leaves the extra resistance gene installed within the Tn10. A major interest of the system is that, once a bacterium carries both Tn10-Tes and Tn10-Tek insertions, a recombination event between the two Tn10 sequences can reconstitute an active tetA gene. This selectable event may be associated with the rearrangement of the sequences surrounding the transposons. This unique property of the "Tes and Tek" system makes it very useful for selection of genomic rearrangements using the Tn10-Tes and Tn10-Tek as predetermined endpoints. The successful isolation of a chromosomal inversion is reported.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:volume
56
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
99-108
pubmed:dateRevised
2000-12-18
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
A system for in vivo selection of genomic rearrangements with predetermined endpoints in Escherichia coli using modified Tn10 transposons.
pubmed:affiliation
Centre de Biochimie et de Génétique cellulaires du C.N.R.S., Université Paul Sabatier, Toulouse, France.
pubmed:publicationType
Journal Article