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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1988-1-14
|
| pubmed:databankReference | |
| pubmed:abstractText |
Human tissue-type plasminogen activator (t-PA) cDNA was cloned from uterine tissue and engineered in expression vectors for production in mouse C127 cells. The vectors consisted of the bovine papilloma virus-1 (BPV-1) genome and t-PA transcriptional unit with a mouse metallothionein (MT-1) promoter at the 5' end and MT-1 genomic sequences or SV40 early introns and polyadenylation signals at the 3' end. Analysis of the expression vectors transfected into cells revealed that t-PA is expressed 100- to 200-fold more with an intronless vector utilizing the SV40 polyadenylation signal than with other, intron-containing vectors. RNA analysis of stable cell lines indicated that t-PA expression levels correlated with mRNA abundance. DNA copy number and transcriptional rate of the MT-1 promoter remained constant in cell lines transformed by different BPV expression vectors. Uterine t-PA produced by recombinant DNA means was enzymatically active and similar in properties to Bowes melanoma t-PA.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0198-0238
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
6
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
461-72
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:2824147-Animals,
pubmed-meshheading:2824147-Base Sequence,
pubmed-meshheading:2824147-Bovine papillomavirus 1,
pubmed-meshheading:2824147-Cloning, Molecular,
pubmed-meshheading:2824147-DNA,
pubmed-meshheading:2824147-Female,
pubmed-meshheading:2824147-Gene Expression Regulation,
pubmed-meshheading:2824147-Genetic Vectors,
pubmed-meshheading:2824147-Humans,
pubmed-meshheading:2824147-Immunosorbent Techniques,
pubmed-meshheading:2824147-Mice,
pubmed-meshheading:2824147-Molecular Sequence Data,
pubmed-meshheading:2824147-Papillomaviridae,
pubmed-meshheading:2824147-Poly A,
pubmed-meshheading:2824147-RNA Splicing,
pubmed-meshheading:2824147-Tissue Plasminogen Activator,
pubmed-meshheading:2824147-Transfection,
pubmed-meshheading:2824147-Uterus
|
| pubmed:year |
1987
|
| pubmed:articleTitle |
Expression of human uterine tissue-type plasminogen activator in mouse cells using BPV vectors.
|
| pubmed:affiliation |
Integrated Genetics, 31 New York Avenue, Framingham, MA 01701.
|
| pubmed:publicationType |
Journal Article
|