Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1987-12-4
pubmed:databankReference
pubmed:abstractText
To better define the function of Saccharomyces cerevisiae SSB1, an abundant single-stranded nucleic acid-binding protein, we determined the nucleotide sequence of the SSB1 gene and compared it with those of other proteins of known function. The amino acid sequence contains 293 amino acid residues and has an Mr of 32,853. There are several stretches of sequence characteristic of other eucaryotic single-stranded nucleic acid-binding proteins. At the amino terminus, residues 39 to 54 are highly homologous to a peptide in calf thymus UP1 and UP2 and a human heterogeneous nuclear ribonucleoprotein. Residues 125 to 162 constitute a fivefold tandem repeat of the sequence RGGFRG, the composition of which suggests a nucleic acid-binding site. Near the C terminus, residues 233 to 245 are homologous to several RNA-binding proteins. Of 18 C-terminal residues, 10 are acidic, a characteristic of the procaryotic single-stranded DNA-binding proteins and eucaryotic DNA- and RNA-binding proteins. In addition, examination of the subcellular distribution of SSB1 by immunofluorescence microscopy indicated that SSB1 is a nuclear protein, predominantly located in the nucleolus. Sequence homologies and the nucleolar localization make it likely that SSB1 functions in RNA metabolism in vivo, although an additional role in DNA metabolism cannot be excluded.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2823109-1270425, http://linkedlifedata.com/resource/pubmed/commentcorrection/2823109-1270426, http://linkedlifedata.com/resource/pubmed/commentcorrection/2823109-1270427, http://linkedlifedata.com/resource/pubmed/commentcorrection/2823109-2414294, http://linkedlifedata.com/resource/pubmed/commentcorrection/2823109-271968, http://linkedlifedata.com/resource/pubmed/commentcorrection/2823109-2985470, http://linkedlifedata.com/resource/pubmed/commentcorrection/2823109-2994041, http://linkedlifedata.com/resource/pubmed/commentcorrection/2823109-2996781, http://linkedlifedata.com/resource/pubmed/commentcorrection/2823109-3001709, http://linkedlifedata.com/resource/pubmed/commentcorrection/2823109-3005291, http://linkedlifedata.com/resource/pubmed/commentcorrection/2823109-3513165, http://linkedlifedata.com/resource/pubmed/commentcorrection/2823109-3518950, http://linkedlifedata.com/resource/pubmed/commentcorrection/2823109-3527040, http://linkedlifedata.com/resource/pubmed/commentcorrection/2823109-3537727, http://linkedlifedata.com/resource/pubmed/commentcorrection/2823109-3733753, http://linkedlifedata.com/resource/pubmed/commentcorrection/2823109-3754960, http://linkedlifedata.com/resource/pubmed/commentcorrection/2823109-3755137, http://linkedlifedata.com/resource/pubmed/commentcorrection/2823109-3763392, http://linkedlifedata.com/resource/pubmed/commentcorrection/2823109-3903656, http://linkedlifedata.com/resource/pubmed/commentcorrection/2823109-3905814, http://linkedlifedata.com/resource/pubmed/commentcorrection/2823109-3941105, http://linkedlifedata.com/resource/pubmed/commentcorrection/2823109-4084504, http://linkedlifedata.com/resource/pubmed/commentcorrection/2823109-4745379, http://linkedlifedata.com/resource/pubmed/commentcorrection/2823109-6096014, http://linkedlifedata.com/resource/pubmed/commentcorrection/2823109-6207644, http://linkedlifedata.com/resource/pubmed/commentcorrection/2823109-6221115, http://linkedlifedata.com/resource/pubmed/commentcorrection/2823109-6235151, http://linkedlifedata.com/resource/pubmed/commentcorrection/2823109-6260570, http://linkedlifedata.com/resource/pubmed/commentcorrection/2823109-6298232, http://linkedlifedata.com/resource/pubmed/commentcorrection/2823109-6365930, http://linkedlifedata.com/resource/pubmed/commentcorrection/2823109-6365931, http://linkedlifedata.com/resource/pubmed/commentcorrection/2823109-6367821, http://linkedlifedata.com/resource/pubmed/commentcorrection/2823109-6575390, http://linkedlifedata.com/resource/pubmed/commentcorrection/2823109-7051968, http://linkedlifedata.com/resource/pubmed/commentcorrection/2823109-7108955
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0270-7306
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2947-55
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Saccharomyces cerevisiae SSB1 protein and its relationship to nucleolar RNA-binding proteins.
pubmed:affiliation
Division of Biology, California Institute of Technology, Pasadena 91125.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't