Linked Life Data

2822717

Source:http://linkedlifedata.com/resource/pubmed/id/2822717

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
31
pubmed:dateCreated
1987-12-10
pubmed:databankReference
pubmed:abstractText
The nucleotide sequence of the operon encoding maize chloroplast ribosomal protein genes S7 and S12 and the promoter activity of a chimeric construct of the -10/-35 sequence of this operon (attached to a promoterless chloramphenicol acetyltransferase gene) have been determined. This operon occurs in the chloroplast genome divided in two parts: part A contains exon 1 of rpS12 (encoding the N-terminal 38 amino acid residues), whereas part B has the following structure: promoter-rpS12 (exon 2 + intron + exon 3)-spacer-rpS7-terminator. Part A is located at the approximate coordinate position 41000, whereas two copies of part B are located at two distant locations in the genome at coordinate positions 18700 and 120200. This unusual organization of the S12 operon in maize (a monocot plant) is similar to that reported in a dicot and a lower plant. The deduced amino acid sequence of maize chloroplast S7 shows 43, 38, 71, and 85% and of S12 shows 66, 72, 91 and 90% sequence identity to the corresponding sequences of Escherichia coli, Euglena gracilis, Marchantia polymorpha, and Nicotiana tabacum, respectively. The promoter upstream of rpS12 (part B) is transcriptionally active in E. coli.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
262
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
15251-5
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Nucleotide sequence, promoter analysis, and linkage mapping of the unusually organized operon encoding ribosomal proteins S7 and S12 in maize chloroplast.
pubmed:affiliation
Max-Planck-Institut für Molekulare Genetik, Abteilung Wittmann, Berlin, West Germany.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.