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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
17
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pubmed:dateCreated |
1987-10-6
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pubmed:abstractText |
Streptococcus lactis metabolizes arginine via the arginine deiminase pathway to ornithine, CO2, NH3, and ATP. The translocation of arginine and ornithine has been studied using membrane vesicles of galactose/arginine-grown cells of S. lactis fused with cytochrome c oxidase proteoliposomes by the freeze/thaw--sonication procedure earlier described. In the presence of reduced cytochrome c the fused membranes rapidly accumulate ornithine. Addition of arginine releases accumulated ornithine. Rapid uncoupler-insensitive exchange between external arginine and internal ornithine is seen at rates that are at least 60-fold higher than the rate of protonmotive force-driven arginine translocation. This arginine:ornithine exchange activity was reconstituted in proteoliposomes after solubilization of S. lactis membranes with octyl beta-D-glucopyranoside. These proteoliposomes catalyze a one-to-one exchange between arginine and ornithine. The arginine:ornithine exchange system is the first exchange system for cationic metabolites found in bacteria. Translocation of arginine via this system does not require metabolic energy obtained by arginine metabolism.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2819865-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2819865-163252,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2819865-2999769,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2819865-3001731,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2819865-3004984,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2819865-3090017,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2819865-3090028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2819865-3108240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2819865-3520229,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2819865-3534538,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2819865-38250,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2819865-6281231,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2819865-6381499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2819865-7000781,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2819865-7003152,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2819865-7056700
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
84
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6093-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1987
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pubmed:articleTitle |
Arginine transport in Streptococcus lactis is catalyzed by a cationic exchanger.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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