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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7
|
| pubmed:dateCreated |
1990-1-5
|
| pubmed:abstractText |
Spindle fiber attachments (SFAs) were enriched in a fraction of nuclei isolated from mouse livers. The enrichment method combines sonication, treatment with 2 mol/L NaCl and high speed centrifugation. SFA was enriched 27-fold on the average (n = 4) when measured by radioimmunoassay. The basic method offers opportunities for further increases of yield and for the enrichment of SFA of other vertebrates.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
1001-652X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
32
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
816-21
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:2818818-Animals,
pubmed-meshheading:2818818-Cell Nucleus,
pubmed-meshheading:2818818-DNA Probes,
pubmed-meshheading:2818818-Immunoglobulin G,
pubmed-meshheading:2818818-Liver,
pubmed-meshheading:2818818-Mice,
pubmed-meshheading:2818818-Mitotic Spindle Apparatus,
pubmed-meshheading:2818818-Scleroderma, Systemic,
pubmed-meshheading:2818818-Transfection
|
| pubmed:year |
1989
|
| pubmed:articleTitle |
Enrichment of the spindle fiber attachments from mouse liver cell nuclei.
|
| pubmed:affiliation |
Shanghai Institute of Cell Biology, Academia Sinica.
|
| pubmed:publicationType |
Journal Article
|