Linked Life Data

2817441

Source:http://linkedlifedata.com/resource/pubmed/id/2817441

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1989-12-6
pubmed:abstractText
Pharmacological and ultrastructural methods were used to demonstrate alpha-adrenergic regulation of secretory granule content of acinar cells of Bowman's glands and to localize and identify adrenergic and cholinergic axonal varicosities and terminals in the olfactory mucosa of the tiger salamander. The alpha-adrenergic agonist phenylephrine caused secretory granule depletion from Bowman's glands; the alpha-adrenergic antagonist phentolamine partially blocked this effect. These observations were quantified using light microscopic computer-assisted morphometric techniques. Both drugs caused morphological signs of electrolye/water transport. Adrenergic axonal varicosities were identified by the presence of small granular vesicles (SGVs, 45-60 nm in diameter) containing electron-dense material that was enhanced by 5-hydroxydopamine loading and chromaffin reaction fixation techniques. Throughout the lamina propria, small fascicles with axons containing SGVs as well as varicosities and terminals with SGVs were located adjacent to blood vessels, Bowman's gland acini, and melanocytes. Mean vesicle diameters at these sites were 54 +/- 7 nm, 50 +/- 9 nm, and 56 +/- 8 nm, respectively; varicosities were located approximately 0.1-1.0 microns from their presumed cellular targets. Axonal varicosities containing small agranular vesicles (AGVs, 65 +/- 8 nm in diameter), identified as cholinergic by their size and by the absence of electron-dense material after 5-hydroxydopamine loading and chromaffin reaction fixation, were located between adjacent acinar cells. In addition, adrenergic varicosities containing SGVs (56 +/- 6 nm in diameter) were found within 1 micron of blood vessels associated with Bowman's gland ducts and sustentacular cells near the base of the olfactory epithelium. These results characterize the ultrastructural basis for adrenergic and cholinergic regulation of vasomotor tone and secretion within the olfactory mucosa.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0003-276X
pubmed:author
pubmed:issnType
Print
pubmed:volume
225
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
232-45
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Ultrastructural localization and identification of adrenergic and cholinergic nerve terminals in the olfactory mucosa.
pubmed:affiliation
Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, Michigan 48201.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.